拟步甲提取物的免疫调节作用。
Immunomodulatory effects of Blaps rynchopetera extract.
机构信息
Master. Dali University - College of Pharmacy - Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D - Dali, China.
PhD. Associate Professor. Dali University - College of Pharmacy - Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D - Dali, China.
出版信息
Acta Cir Bras. 2022 May 2;37(2):e370205. doi: 10.1590/acb370205. eCollection 2022.
PURPOSE
To explore the potential immunomodulatory effects of total extract and different polar parts from Blaps rynchopetera Fairmaire.
METHODS
Phagocytic activity was evaluated by neutral red assay, and the effect of the immune function was investigated by normal and immunocompromised mice models.
RESULTS
In vitro, total extract, as well as chloroform, ethyl acetate, n-butanol and water fractions could individually enhance the phagocytic ability of mouse peritoneal macrophages; in addition, chloroform and ethyl acetate fractions had an increasing tendency when combined stimulation with lipopolysaccharide (LPS). In vivo, ethyl acetate fraction (EAF) could enhance the immune organ index, increase the serum hemolysin level and peripheral blood immune cells of immunocompromised mice, while for normal mice, the effect was inconspicuous.
CONCLUSIONS
Blaps rynchopetera extracts had noteworthy immunomodulatory effect, especially for individuals with immune disorders.
目的
探讨阳彩臂金龟总提物及其不同极性部位的潜在免疫调节作用。
方法
采用中性红法评价吞噬活性，通过正常和免疫抑制小鼠模型研究免疫功能的影响。
结果
体外实验中，总提物以及氯仿、乙酸乙酯、正丁醇和水相均可单独增强小鼠腹腔巨噬细胞的吞噬能力；此外，氯仿和乙酸乙酯相在与脂多糖（LPS）联合刺激时呈递增趋势。体内实验中，乙酸乙酯相（EAF）可增强免疫抑制小鼠的免疫器官指数，提高血清溶血素水平和外周血免疫细胞，而对正常小鼠的作用不明显。
结论
阳彩臂金龟提取物具有显著的免疫调节作用，尤其对免疫功能紊乱的个体。
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