Analyzing the Distribution of Specialized Metabolites from Plant Native Tissues with Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging.

The localization of metabolites in plant tissues is often related to their biological function and biosynthesis. Mass spectrometry imaging (MSI) provides comprehensive information about the distribution of known and unknown compounds in tissues. In this protocol, we describe the use of laser desorption low-temperature plasma (LD-LTP) ionization MSI. This technology enables the direct analysis of native tissues under ambient conditions.