Feng Baosheng, Zhang Jialing, Chang Cuilan, Li Liping, Li Min, Xiong Xingchuang, Guo Chengan, Tang Fei, Bai Yu, Liu Huwei
Beijing National Laboratory for Molecular Sciences, the Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Anal Chem. 2014 May 6;86(9):4164-9. doi: 10.1021/ac403310k. Epub 2014 Apr 28.
Mass spectrometry imaging (MSI) has been widely used in many research areas for the advantages of providing informative molecular distribution with high specificity. Among the recent progress, ambient MSI has attracted increasing interests owing to its characteristics of ambient, in situ, and nonpretreatment analysis. Here, we are presenting the ambient MSI for traditional Chinese medicines (TCMs) and authentication of work of art and documents using plasma assisted laser desorption ionization mass spectrometry (PALDI-MS). Compared with current ambient MSI methods, an excellent average resolution of 60 μm × 60 μm pixel size was achieved using this system. The feasibility of PALDI-based MSI was confirmed by seal imaging, and its authentication applications were demonstrated by imaging of printed Chinese characters. Imaging of the Radix Scutellariae slice showed that the two active components, baicalein and wogonin, mainly were distributed in the epidermis of the root, which proposed an approach for distinguishing TCMs' origins and the distribution of active components of TCMs and exploring the environmental effects of plant growth. PALDI-MS imaging provides a strong complement for the MSI strategy with the enhanced spatial resolution, which is promising in many research fields, such as artwork identification, TCMs' and botanic research, pharmaceutical applications, etc.
质谱成像（MSI）因其能够提供具有高特异性的丰富分子分布信息的优势，已在许多研究领域中得到广泛应用。在最近的进展中，常压质谱成像因其常压、原位和无需预处理分析的特点而引起了越来越多的关注。在此，我们展示了使用等离子体辅助激光解吸电离质谱（PALDI-MS）对中药以及艺术品和文件进行鉴定的常压质谱成像。与当前的常压质谱成像方法相比，该系统实现了60μm×60μm像素尺寸的出色平均分辨率。基于PALDI的质谱成像的可行性通过印章成像得到证实，其鉴定应用通过印刷汉字成像得以展示。黄芩切片成像表明，两种活性成分黄芩素和汉黄芩素主要分布在根的表皮中，这为区分中药的产地和中药活性成分的分布以及探索植物生长的环境影响提供了一种方法。PALDI-MS成像以增强的空间分辨率为质谱成像策略提供了有力补充，在许多研究领域，如艺术品鉴定、中药和植物研究、药物应用等方面具有广阔前景。
