Ikeda Yoshito, Munekane Masayuki, Yamada Yasuyuki, Kawakami Mizuki, Amano Ikuko, Sano Kohei, Mukai Takahiro, Kambe Taiho, Shitan Nobukazu
Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University, Kobe, Japan.
Laboratory of Biophysical Chemistry, Kobe Pharmaceutical University, Kobe, Japan.
J Ginseng Res. 2022 Mar;46(2):248-254. doi: 10.1016/j.jgr.2021.06.006. Epub 2021 Jun 15.
Zinc homeostasis is essential for human health and is regulated by several zinc transporters including ZIP and ZnT. ZIP4 is expressed in the small intestine and is important for zinc absorption from the diet. We investigated in the present study the effects of () extract on modulating Zip4 expression and cellular zinc levels in mouse Hepa cells.
Hepa cells were transfected with a luciferase reporter plasmid that contains metal-responsive elements, incubated with extract, and luciferase activity was measured. Using ZnCl, zinc uptake in -treated cells was measured. The expression of Zip4 mRNA and protein in Hepa cells was also investigated. Finally, using a luciferase reporter assay system, the effects of several ginsenosides were monitored.
The luciferase activity in cells incubated with extract was significantly higher than that of control cells cultured in normal medium. Hepa cells treated with extract exhibited higher zinc uptake. extract induced mRNA expression, which resulted in an enhancement of Zip4 protein expression. Furthermore, some ginsenosides, such as ginsenoside Rc and Re, enhanced luciferase activity driven by intracellular zinc levels.
extract induced Zip4 expression at the mRNA and protein level and resulted in higher zinc uptake in Hepa cells. Some ginsenosides facilitated zinc influx. On the basis of these results, we suggest a novel effect of on Zip4-mediated zinc influx, which may provide a new strategy for preventing zinc deficiency.
锌稳态对人体健康至关重要，且受包括ZIP和ZnT在内的多种锌转运蛋白调节。ZIP4在小肠中表达，对饮食中锌的吸收很重要。我们在本研究中调查了（）提取物对小鼠Hepa细胞中Zip4表达和细胞锌水平的调节作用。
用含有金属反应元件的荧光素酶报告质粒转染Hepa细胞，与提取物一起孵育，然后测量荧光素酶活性。使用ZnCl，测量提取物处理细胞中的锌摄取。还研究了Hepa细胞中Zip4 mRNA和蛋白质的表达。最后，使用荧光素酶报告分析系统，监测几种人参皂苷的作用。
与提取物孵育的细胞中的荧光素酶活性显著高于在正常培养基中培养的对照细胞。用提取物处理的Hepa细胞表现出更高的锌摄取。提取物诱导mRNA表达，从而导致Zip4蛋白表达增强。此外，一些人参皂苷，如人参皂苷Rc和Re，增强了由细胞内锌水平驱动的荧光素酶活性。
提取物在mRNA和蛋白质水平上诱导Zip4表达，并导致Hepa细胞中更高的锌摄取。一些人参皂苷促进锌内流。基于这些结果，我们提出提取物对Zip4介导的锌内流有新的作用，这可能为预防锌缺乏提供新策略。
