Radiotherapy Center, Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi, China.
Department of Oncology No. 2, Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi, China.
Cancer Gene Ther. 2017 Sep;24(9):361-366. doi: 10.1038/cgt.2017.25. Epub 2017 Jul 28.
Sijunzi decoction (SJZD) is a traditional Chinese medicine prescription for the prevention and treatment of colorectal cancer. Our study is aimed to identify the potential therapeutic target of SJZD. Microarray analysis was performed on the patients with colorectal cancer under treatment of SJZD. The mRNA targets of SJZD were predicted by BATMAN-TCM database and were further identified by integrated analysis of expression data from TCGA. The correlation of gene expression and clinical characteristics was analyzed. Microarray analysis indicated that Krüppel-like factor 4 (KLF4) was significantly upregulated after intervention of SJZD. BATMAN-TCM prediction showed that KLF4 was an mRNA target of SJZD and it was downregulated in colorectal cancer by integrated analysis of expression data from TCGA. KLF4 may act as an administered indicator to assess whether adjuvant postoperative pharmaceutical therapy is needed for patients with colorectal cancer. Low KLF4 expression was significantly correlated with reductions of overall survival and recurrence rate. Our results suggested that KLF4 may be the potential therapeutic target of SJZD for the treatment of colorectal cancer.
四君子汤（SJZD）是一种用于预防和治疗结直肠癌的中药方剂。我们的研究旨在确定 SJZD 的潜在治疗靶点。对接受 SJZD 治疗的结直肠癌患者进行了微阵列分析。通过 BATMAN-TCM 数据库预测 SJZD 的 mRNA 靶点，并通过整合 TCGA 的表达数据进一步鉴定。分析了基因表达与临床特征的相关性。微阵列分析表明，干预 SJZD 后 Krüppel 样因子 4（KLF4）显著上调。BATMAN-TCM 预测显示，KLF4 是 SJZD 的 mRNA 靶点，通过整合 TCGA 的表达数据进行分析，KLF4 在结直肠癌中下调。KLF4 可能作为评估结直肠癌患者是否需要辅助术后药物治疗的给药指标。低 KLF4 表达与总生存率和复发率的降低显著相关。我们的研究结果表明，KLF4 可能是 SJZD 治疗结直肠癌的潜在治疗靶点。
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