Laboratory Medicine Center, Huazhong University of Science and Technology Union Shenzhen Hospital and the 6th Affiliated Hospital of Shenzhen University, Guangdong Shenzhen 518082, China.
Heilongjiang Medical Service Management Evaluation Center, Heilongjiang, Haerbin 150081, China.
Comput Intell Neurosci. 2022 Apr 25;2022:7636983. doi: 10.1155/2022/7636983. eCollection 2022.
This study aimed to evaluate the activity, resistance, clonality of MIC distribution, and the correlation between virulence and resistance genes and biofilm formation of omadacycline (OMC) in clinics for isolates from China. 162 isolates were collected retrospectively in China. The were collected from the body's cervical secretions, wound secretions, ear swabs, secretions, semen, venous blood, cerebrospinal fluid, pee, etc. The MIC of OMC against was determined by broth microdilution. The inhibition zone diameters of OMC and other common antibiotics were measured using filter paper. D-test was performed to determine the phenotype of cross resistance between erythromycin and clindamycin. In Multilocus sequence typing (MLST), some commonly-detected resistance genes and virulence gene of these isolates were investigated using polymerase chain reaction (PCR). Biofilms were detected by crystal violet staining. Our data demonstrated the correalation of the biofilm formation and OMA antimicrobial susceptibility of S.agalactiae clinical isolates with the carrier of virulence gene scpB. Conclusively, OMC exhibits the robust antimcirobial activity against clinical S. agalactiae isolates from China compared with DOX or MIN, and the carrier of the virulence gene scpB might correlate with the biofilm formation in OMC-resistant .
本研究旨在评估临床分离的 162 株来自中国的 对奥马环素（OMC）的活性、耐药性、MIC 分布的克隆性，以及毒力基因与耐药基因和生物膜形成之间的相关性。这些 是从人体宫颈分泌物、伤口分泌物、耳拭子、分泌物、精液、静脉血、脑脊液、尿液等部位采集的。采用肉汤微量稀释法测定 OMC 对 的 MIC。采用滤纸片法测定 OMC 与其他常用抗生素的抑菌圈直径。采用 D 试验测定红霉素和克林霉素交叉耐药表型。采用聚合酶链反应（PCR）对这些 分离株的一些常见耐药基因和毒力基因进行多基因序列分型（MLST）分析。采用结晶紫染色法检测生物膜。我们的数据表明，携带毒力基因 scpB 的 临床分离株的生物膜形成与 OMA 抗菌药物敏感性之间存在相关性。结论：与 DOX 或 MIN 相比，OMC 对来自中国的临床 具有更强的抗菌活性，携带毒力基因 scpB 可能与 OMC 耐药 中的生物膜形成有关。
