J Biomed Nanotechnol. 2020 Mar 1;16(3):390-397. doi: 10.1166/jbn.2020.2896.
KSL-W peptide has demonstrated antibacterial and antifungal activity and inhibitory effects against oral biofilm. This study aimed to check out the effect of chlorhexidine (CLX) or KSL-W peptide-loaded poloxamer 407-based microemulsions for buccal delivery on () biofilm. The formulation () containing 10% copolymer poloxamer 407 dispersion (1%), 40% oleic acid and 50% PPG-5-CETETH-20 was characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, bioadhesive and syringeability; and in the treatment of a biofilm produced by . The darkfield images obtained by PLM and the SAXS curves with an extended peak indicated that the system was characteristic of microemulsions. In a continuous analysis, microemulsions exhibited Newtonian behavior. In frequency, the oscillatory analysis profile presented predominantly viscous behavior. Bioadhesive force detected in the analysis of F (7.4 ± 1.81 mN˙ s) and syringeability (17.83± 5.97 N · mm) being adequate values for buccal administration. After 4 h, KSL-W-loaded F shown over 20% higher effectiveness than chlorhexidine-loaded microemulsions. In conclusion, the KSL-W-loaded microemulsions showed a considerable reduction in biofilm formation and presented promising structural properties for buccal drug delivery.
KSL-W 肽具有抗菌和抗真菌活性，并对口腔生物膜具有抑制作用。本研究旨在考察氯己定（CLX）或载 KSL-W 肽的泊洛沙姆 407 基微乳用于颊部给药对 （）生物膜的影响。该配方（）含有 10%共聚物泊洛沙姆 407 分散体（1%）、40%油酸和 50%PPG-5-CETETH-20，通过偏光显微镜（PLM）、小角 X 射线散射（SAXS）、流变学、生物黏附性和可推注性进行了表征；并在治疗 产生的生物膜方面进行了研究。PLM 获得的暗场图像和具有扩展峰的 SAXS 曲线表明该系统具有微乳液的特征。在连续分析中，微乳液表现出牛顿行为。在频率上，振荡分析曲线呈现出主要的粘性行为。在 F（7.4±1.81 mN˙s）和可推注性（17.83±5.97 N·mm）的分析中检测到的生物黏附力是颊部给药的合适值。在 4 小时后，载 KSL-W 的 F 显示出比载氯己定的微乳高出 20%以上的有效性。总之，载 KSL-W 的微乳显示出对 生物膜形成有相当大的减少作用，并表现出用于颊部药物输送的有前途的结构特性。
