Instituto de Productos Naturales y Agrobiología (IPNA-CSIC), Avda. Astrofísico Fco. Sánchez 3, 38206, La Laguna, Spain.
GIR MIOMeT-IU Cinquima-Química Inorgánica, Facultad de Ciencias, Campus Miguel Delibes, Universidad de Valladolid, 47011, Valladolid, Spain.
Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202205403. doi: 10.1002/anie.202205403. Epub 2022 May 23.
3-Nitrotyrosine (NT) is generated by the action of peroxynitrite and other reactive nitrogen species (RNS), and as a consequence it is accumulated in inflammation-associated conditions. This is particularly relevant in kidney disease, where NT concentration in blood is considerably high. Therefore, NT is a crucial biomarker of renal damage, although it has been underestimated in clinical diagnosis due to the lack of an appropriate sensing method. Herein we report the first fluorescent supramolecular sensor for such a relevant compound: Fluorescence by rotational restriction of tetraphenylethenes (TPE) in a covalent cage is selectively quenched in human blood serum by 3-nitrotyrosine (NT) that binds to the cage with high affinity, allowing a limit of detection within the reported physiological concentrations of NT in chronic kidney disease.
3-硝基酪氨酸 (NT) 是由过氧亚硝酸盐和其他活性氮物种 (RNS) 的作用产生的，因此它在炎症相关的情况下积累。这在肾脏疾病中尤为重要，因为血液中的 NT 浓度相当高。因此，NT 是肾脏损伤的一个关键生物标志物，尽管由于缺乏适当的传感方法，在临床诊断中被低估了。在这里，我们报告了第一个用于这种相关化合物的荧光超分子传感器：在共价笼中通过旋转限制四苯乙烯 (TPE) 的荧光被 3-硝基酪氨酸 (NT) 选择性猝灭，NT 以高亲和力与笼结合，允许在慢性肾病中报告的 NT 的生理浓度范围内进行检测。
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