Rousseaux-Prevost R, Capron M, Bazin H, Capron A
Immunology. 1978 Jul;35(1):33-9.
Parasite specific IgE antibodies in rats infected with Schistosoma manoni were measured by passive cutaneous anaphylaxis (PCA) reactions and by the technique of immuno-adsorption. Two strains, one a low IgE producer (Fischer rats) and the other a high IgE producer (Hooded-Lister rats) were studied. In Fischer rats, a time course study of the occurrence of IgE antibodies and resistance to reinfection was made. Parasite specific IgE levels measured by immuno-adsorpiton were much lower than total IgE levels and a similar percentage of specific IgE (about 8%) was in the two strains.IgE antibodies were maximum at day 30 and day 60 after infection; however, a third peak at day 90 was observed only in Fischer rats. Some discrepancies between results obtained by PCA and immunosorbent techniques have been observed, which could be explained by differences in the affinity of IgE antibodies during infection or by the presence of total IgE in the PCA assay. There was a close parallelism between specific IgE antibodies levels and the course of immunity in Fischer rats. This parallelism supports the view that IgE could play a pre-eminent role in protective immunity in rat schistosomiasis.
通过被动皮肤过敏反应（PCA）和免疫吸附技术，对感染曼氏血吸虫的大鼠体内的寄生虫特异性IgE抗体进行了检测。研究了两个品系的大鼠，一个是低IgE产生者（Fischer大鼠），另一个是高IgE产生者（Hooded-Lister大鼠）。对Fischer大鼠进行了IgE抗体出现情况和再感染抵抗力的时间进程研究。通过免疫吸附法测得的寄生虫特异性IgE水平远低于总IgE水平，且两个品系中特异性IgE的比例相似（约8%）。感染后第30天和第60天IgE抗体水平最高；然而，仅在Fischer大鼠中观察到感染后第90天出现第三个峰值。已观察到PCA和免疫吸附技术所得结果之间存在一些差异，这可能是由于感染期间IgE抗体亲和力的差异或PCA检测中总IgE的存在所致。Fischer大鼠体内特异性IgE抗体水平与免疫进程密切相关。这种相关性支持了IgE在大鼠血吸虫病保护性免疫中可能起重要作用的观点。
