Pei Yong, Si Jierui, Navet Natasha, Ji Peiyun, Zhang Xiong, Qiao Huijun, Xu Ruofei, Zhai Ying, Miao Jianqiang, Tyler Brett M, Dou Daolong
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, United States.
Fungal Genet Biol. 2022 Jul;161:103695. doi: 10.1016/j.fgb.2022.103695. Epub 2022 May 2.
Being found in all eukaryotes investigated, acyl-CoA-binding proteins (ACBPs) participate in lipid metabolism via specifically binding acyl-CoA esters with high affinity. The structures and functions of ACBP family proteins have been extensively described in yeasts, fungi, plants and mammals, but not oomycetes. In the present study, seven ACBP genes named PsACBP1-7 were identified from the genome of Phytophthora sojae, an oomycete pathogen of soybean. CRISPR-Cas9 knockout mutants targeting PsACBP1 and PsACBP2 were created for phenotypic assays. PsACBP1 knockout led to defects in sporangia production and virulence. PsACBP2 knockout mutants exhibited impaired vegetative growth, zoospore production, cyst germination and virulence. Moreover, Nile red staining of PsACBP2 knockout and over-expression lines showed that PsACBP2 is involved in the formation of lipid bodies in P. sojae. Our results demonstrate that two ACBP genes are differently required for growth and development, and both are essential for virulence in P. sojae.
在所有已研究的真核生物中均发现，酰基辅酶A结合蛋白（ACBPs）通过特异性高亲和力结合酰基辅酶A酯来参与脂质代谢。ACBP家族蛋白的结构和功能在酵母、真菌、植物和哺乳动物中已有广泛描述，但卵菌中尚未见报道。在本研究中，从大豆卵菌病原体大豆疫霉的基因组中鉴定出7个名为PsACBP1 - 7的ACBP基因。构建了靶向PsACBP1和PsACBP2的CRISPR - Cas9敲除突变体用于表型分析。PsACBP1敲除导致孢子囊产生和毒力缺陷。PsACBP2敲除突变体表现出营养生长、游动孢子产生、孢囊萌发和毒力受损。此外，对PsACBP2敲除和过表达株系的尼罗红染色表明，PsACBP2参与大豆疫霉脂质体的形成。我们的结果表明，两个ACBP基因在生长和发育中需求不同，且二者对大豆疫霉的毒力均至关重要。
