Zhuang Jing, Zhang Ya-Duo, Sun Wei-Xia, Zong Juan, Li Jun-Jiao, Dai Xiao-Feng, Klosterman Steven J, Chen Jie-Yin, Tian Li, Subbarao Krishna V, Zhang Dan-Dan
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193 China.
School of Life Science, Qufu Normal University, Qufu, 273165 China.
aBIOTECH. 2024 Jul 13;5(4):431-448. doi: 10.1007/s42994-024-00175-3. eCollection 2024 Dec.
In the face of carbon, nitrogen, and phosphorus starvation, microorganisms have evolved adaptive mechanisms to maintain growth. In a previous study, we identified a protein predicted to contain acyl-CoA-binding domains in the plant pathogenic fungus . The predicted protein, designated VdAcb1, possesses an atypical signal peptide. However, the functions of this acyl-CoA-binding protein in are not clear. In this research, in vivo or in vitro assays confirmed that VdAcb1 is secreted extracellularly from , although it does not have the typical signal peptide. Furthermore, the unconventional secretion of VdAcb1 was dependent on VdGRASP, a member of the compartment for unconventional protein secretion (CUPS). The deletion mutant strain of (Δ exhibited significant sensitivity to carbon starvation. RNA-seq revealed that the expression of genes related to filamentous growth (MSB2 pathway) and sugar transport were regulated by under conditions of carbon starvation. Yeast one-hybrid experiments further showed that the expression of VdAcb1 was positively regulated by the transcription factor VdMsn4. The Δ strain showed significantly reduced virulence on and . We hypothesize that under conditions of carbon starvation, the expression of is activated by VdMsn4 and VdAcb1 is secreted into the extracellular space. In turn, this activates the downstream MAPK pathway to enhance filamentous growth and virulence of .
The online version contains supplementary material available at 10.1007/s42994-024-00175-3.
面对碳、氮和磷饥饿，微生物进化出了维持生长的适应性机制。在之前的一项研究中，我们在植物致病真菌中鉴定出一种预测含有酰基辅酶A结合结构域的蛋白质。该预测蛋白命名为VdAcb1，具有非典型信号肽。然而，这种酰基辅酶A结合蛋白在[具体真菌名称未给出]中的功能尚不清楚。在本研究中，体内或体外试验证实VdAcb1从[具体真菌名称未给出]细胞外分泌，尽管它没有典型信号肽。此外，VdAcb1的非常规分泌依赖于VdGRASP，它是非常规蛋白质分泌区室（CUPS）的成员。[具体真菌名称未给出]的缺失突变菌株（Δ[具体名称未给出]）对碳饥饿表现出显著敏感性。RNA测序显示，在碳饥饿条件下，与丝状生长（MSB2途径）和糖转运相关的基因表达受[具体真菌名称未给出]调控。酵母单杂交实验进一步表明，转录因子VdMsn4对VdAcb1的表达具有正向调控作用。Δ[具体名称未给出]菌株在[具体植物名称未给出]和[具体植物名称未给出]上的毒力显著降低。我们假设在碳饥饿条件下，[具体真菌名称未给出]的表达被VdMsn4激活，VdAcb1分泌到细胞外空间。反过来，这激活下游丝裂原活化蛋白激酶（MAPK）途径，以增强[具体真菌名称未给出]的丝状生长和毒力。
在线版本包含可在10.1007/s42994-024-00175-3获取的补充材料。
