Zhang Baiyu, Zhang Zhichao, Yong Saijiang, Yu Shuyang, Feng Hui, Yin Maozhu, Ye Wenwu, Wang Yuanchao, Qiu Min
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
The Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Phytopathology. 2022 Nov;112(11):2351-2359. doi: 10.1094/PHYTO-12-21-0523-R. Epub 2022 Nov 30.
A leucine-rich repeat (LRR) is a widespread structural motif of 20 to 30 amino acids with characteristic repetitive sequences rich in leucine. LRR-containing proteins are critical for ligand recognition and binding, participating in plant development and defense. Like plants, oomycetes also harbor genes encoding LRR-containing proteins, but their functions remain largely unknown. We identified a zoospore-upregulated gene from with LRRs and an extra structural maintenance of chromosomes-like domain. We generated knockout and complemented knockout strains of this LRR protein and found that its deletion resulted in a pronounced reduction in zoospore mobility and chemotaxis, cyst germination, and virulence. Interestingly, micro-examination of zoospores under a scanning electron microscope revealed irregularly shaped zoospores without flagella in these deletion mutants. In addition, the reintroduction of this LRR protein into the knockout mutant reversed all the deficiencies. Our data demonstrate a critical role for the LRR protein in modulating zoospore development, which impairs migration to the host soybean and affects the spread of pathogens.
富含亮氨酸重复序列（LRR）是一种广泛存在的结构基序，由20至30个氨基酸组成，具有富含亮氨酸的特征性重复序列。含LRR的蛋白质对于配体识别和结合至关重要，参与植物发育和防御。与植物一样，卵菌也含有编码含LRR蛋白质的基因，但其功能在很大程度上仍不清楚。我们从[具体来源]中鉴定出一个游动孢子上调基因，该基因具有LRR和一个额外的类染色体结构维持结构域。我们构建了该LRR蛋白的敲除和互补敲除菌株，发现其缺失导致游动孢子的运动性和趋化性、孢囊萌发及毒力显著降低。有趣的是，在扫描电子显微镜下对游动孢子进行微观检查发现，这些缺失突变体中的游动孢子形状不规则且无鞭毛。此外，将该LRR蛋白重新导入敲除突变体中可逆转所有缺陷。我们的数据证明了该LRR蛋白在调节游动孢子发育中起关键作用，这会损害其向宿主大豆的迁移并影响病原体的传播。
