Wang Na, Gao Jin, Liu Yang, Shi Rui, Chen Songlin
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, China.
Hainan Academy of Ocean and Fisheries Sciences, Haikou 570203, China.
Genomics. 2022 May;114(3):110376. doi: 10.1016/j.ygeno.2022.110376. Epub 2022 May 2.
Sexual size dimorphism (SSD), whereby females and males exhibit different body sizes, are widely documented in animals. To explore crucial regulators implicated in female-biased SSD of Chinese tongue sole (Cynoglossus semilaevis), GWAS was conducted on 350 females and 59 males. Twenty SNPs and 25 genes including zbed1, nsd3, cdc45, klhl29, and smad4 with -log(p) > 7 were screened, mainly mapping to sex chromosome. The chromosome W-linked gene zbed1 attracted particular attention because it is a master key for cell proliferation. Thus, the regulatory network of zbed1 in C. semilaevis was explored by DAP-seq and 1352 peaks were discovered in the female brain. Moreover, zbed1 potentially regulated hippo signaling pathway, cell cycle, translation, and PI3k-Akt signaling pathway in C. semilaevis. These findings identify crucial SNPs and genes associated with female-biased SSD in C. semilaevis, also provide the first genome-wide survey for the zbed1 regulatory network in fish species.
两性体型差异（SSD），即雌性和雄性表现出不同的体型，在动物中广泛存在。为了探究与半滑舌鳎（Cynoglossus semilaevis）雌性偏向的SSD相关的关键调控因子，对350只雌性和59只雄性进行了全基因组关联研究（GWAS）。筛选出了20个单核苷酸多态性（SNP）和25个基因，包括zbed1、nsd3、cdc45、klhl29和smad4，其-log(p) > 7，主要定位在性染色体上。染色体W连锁基因zbed1引起了特别关注，因为它是细胞增殖的关键因子。因此，通过DNA亲和纯化测序（DAP-seq）探索了半滑舌鳎中zbed1的调控网络，并在雌性大脑中发现了1352个峰。此外，zbed1可能调控半滑舌鳎中的河马信号通路、细胞周期、翻译和PI3k-Akt信号通路。这些发现确定了与半滑舌鳎雌性偏向的SSD相关的关键SNP和基因，也为鱼类中zbed1调控网络提供了首次全基因组调查。
