Molecular Mechanism of the Gene Family Regulating Growth Size Heteromorphism in .

There are obvious individual differences in the growth and development of , mainly due to female bias. We selected 500 female of different sizes for GWAS and transcriptome analysis to screen for differential genes. qPCR was performed to detect the expression of the genes in various tissues, and RNAi experiments were performed in testicular cells to knock down the and genes and transcriptome sequencing was performed to check the changes of the downstream genes. gene was screened for the common genes by GWAS sequencing and transcriptome sequencing. In the QPCR results, the expression of the gene family was negatively correlated with fish size, and was slightly higher in males than in females; in the transcriptome results, the expression of , , , and was up-regulated, and the expression of was down-regulated. It is speculated that has an antagonistic effect on , which is deregulated when the gene family is knocked down. The gene family may affect the growth of individual through the gh-igf1 axis, which provides a basis for the study of the differences in the growth size of