Wang Yaning, Chen Yadong, Liu Yang, Chen Songlin
College of Life Science, Qingdao University, Qingdao 266071, China.
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Animals (Basel). 2025 Apr 14;15(8):1130. doi: 10.3390/ani15081130.
There are obvious individual differences in the growth and development of , mainly due to female bias. We selected 500 female of different sizes for GWAS and transcriptome analysis to screen for differential genes. qPCR was performed to detect the expression of the genes in various tissues, and RNAi experiments were performed in testicular cells to knock down the and genes and transcriptome sequencing was performed to check the changes of the downstream genes. gene was screened for the common genes by GWAS sequencing and transcriptome sequencing. In the QPCR results, the expression of the gene family was negatively correlated with fish size, and was slightly higher in males than in females; in the transcriptome results, the expression of , , , and was up-regulated, and the expression of was down-regulated. It is speculated that has an antagonistic effect on , which is deregulated when the gene family is knocked down. The gene family may affect the growth of individual through the gh-igf1 axis, which provides a basis for the study of the differences in the growth size of
在……的生长和发育过程中存在明显的个体差异，主要是由于雌性偏向。我们选择了500只不同大小的雌性……进行全基因组关联研究（GWAS）和转录组分析，以筛选差异基因。进行定量聚合酶链反应（qPCR）来检测这些基因在各种组织中的表达，并在睾丸细胞中进行RNA干扰（RNAi）实验以敲低……基因，然后进行转录组测序以检查下游基因的变化。通过GWAS测序和转录组测序筛选出……基因作为共同基因。在qPCR结果中，……基因家族的表达与鱼的大小呈负相关，且在雄性中略高于雌性；在转录组结果中，……、……、……和……的表达上调，而……的表达下调。推测……对……有拮抗作用，当……基因家族被敲低时该作用失调。……基因家族可能通过生长激素-胰岛素样生长因子1（gh-igf1）轴影响个体……的生长，这为研究……生长大小差异提供了依据
