Department of Ophthalmology, Keimyung University School of Medicine, Dongsan Medical Center, Daegu, Korea.
Sci Rep. 2022 May 5;12(1):7331. doi: 10.1038/s41598-022-10994-3.
This study sought to describe the use of deep-frozen donor corneal remnants preserved after keratoplasty procedures for therapeutic or tectonic keratoplasty without subsequent optical keratoplasty. This single-center retrospective consecutive case series analyzed the electronic medical records of patients who had undergone therapeutic or tectonic keratoplasty using deep-frozen donor remains preserved in Optisol-GS, for the past 11 years at Keimyung University Dongsan Medical Center. Fifty-five surgical cases in 46 patients were included. Twenty-three surgical cases in 18 patients underwent therapeutic keratoplasty for refractory infectious corneal ulcer. Complete eradication of primary infection was achieved in 14 patients (77.8%). Tectonic keratoplasty were performed 32 cases in 28 patients. Twenty-seven of 28 patients were ultimately able to maintain anatomical integrity (96.4%). Mean uncorrected visual acuity improved from 1.77 ± 0.94 preoperatively to 1.31 ± 0.95 at the last follow-up postoperatively in the tectonic graft group by logarithm of the minimal angle of resolution (P = 0.002). There were no cases of graft rejection. Keratoplasty using cryopreserved donor tissue is a suitable surgical alternative for infectious or non-infectious corneal ulcers in elderly patients or patients with poor general condition. It could be a viable alternative to overcome the shortage of corneal donors.
本研究旨在描述在穿透性角膜移植术后使用深低温保存的供体角膜残余物进行治疗性或结构性角膜移植术，而不进行后续光学性角膜移植术的情况。本单中心回顾性连续病例系列研究分析了过去 11 年在全州大学东山医疗中心使用 Optisol-GS 保存的深低温保存的供体残余物进行治疗性或结构性角膜移植术的患者的电子病历。共纳入 46 例患者的 55 例手术。18 例患者的 23 例手术接受了难治性感染性角膜溃疡的治疗性角膜移植术。14 例患者（77.8%）原发性感染完全消除。28 例患者中有 32 例接受了结构性角膜移植术。28 例患者中有 27 例最终能够保持解剖完整性（96.4%）。结构性移植物组中，未矫正视力从术前的 1.77±0.94 提高到术后最后一次随访时的 1.31±0.95，平均对数视力（P=0.002）。没有移植物排斥的病例。对于老年患者或一般状况较差的患者的感染性或非感染性角膜溃疡，使用冷冻保存的供体组织进行角膜移植术是一种合适的手术选择。它可能是克服角膜供体短缺的可行替代方法。
