Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, 87#, Dingjiaqiao Road, Gulou District, Nanjing City, Jiangsu Province, China.
Maternal and Child Health Center of Gulou District, Nanjing, China.
BMC Microbiol. 2022 May 5;22(1):121. doi: 10.1186/s12866-022-02545-7.
To explore the impact of pre-pregnancy vaginal Mycoplasma hominis (M. hominis) colonization of low abundance on female fecundability.
In total, 89 females participating in a pre-pregnancy health examination program were included, and their pregnancy outcomes were followed up for 1 year. Vaginal swabs were collected, 16S rRNA genes were sequenced, and M. hominis colonization was confirmed by qPCR. Cox models were used to estimate the fecundability odds ratio (FOR) for women with M. hominis.
The prevalence of M. hominis was 22.47% (20/89), and the abundance was relatively low (the cycle thresholds of the qPCR were all more than 25). In terms of the vaginal microbiome, the Simpson index of the positive group was significantly lower than that of the negative group (P = 0.003), which means that the microbiome diversity appeared to increase with M. hominis positivity. The relative abundance of M. hominis was negatively correlated with Lactobacillus crispatus (rho = - 0.24, P = 0.024), but positively correlated with Gardnerella vaginalis, Atopobium vaginae and Prevotella bivia (P all < 0.05). The cumulative one-year pregnancy rate for the M. hominis positive group was lower than that in the negative group (58.96% vs 66.76%, log-rank test: P = 0.029). After controlling for potential confounders, the risk of pregnancy in the M. hominis positive group was reduced by 38% when compared with the positive group (FOR = 0.62, 95% CI: 0.42-0.93).
The vaginal colonization of M. hominis at a low level in pre-pregnant women is negatively correlated with female fecundability.
探讨孕前阴道低丰度人型支原体（M. hominis）定植对女性生育能力的影响。
共纳入 89 名参加孕前健康检查计划的女性，随访其妊娠结局 1 年。采集阴道拭子，进行 16S rRNA 基因测序，并通过 qPCR 确认 M. hominis 定植。采用 Cox 模型估计 M. hominis 阳性女性的生育能力比值比（FOR）。
M. hominis 的检出率为 22.47%（20/89），丰度较低（qPCR 的循环阈值均超过 25）。就阴道微生物组而言，阳性组的 Simpson 指数明显低于阴性组（P=0.003），这意味着 M. hominis 阳性组的微生物组多样性似乎随着 M. hominis 阳性而增加。M. hominis 的相对丰度与乳酸杆菌 crispatus 呈负相关（rho=-0.24，P=0.024），但与加德纳菌、阴道阿托波菌和普雷沃氏菌呈正相关（P 均<0.05）。M. hominis 阳性组的 1 年累积妊娠率低于阴性组（58.96% vs 66.76%，对数秩检验：P=0.029）。在校正潜在混杂因素后，M. hominis 阳性组的妊娠风险较阴性组降低 38%（FOR=0.62，95%CI：0.42-0.93）。
孕前女性阴道低水平的 M. hominis 定植与女性生育能力呈负相关。
