[小鼠B细胞杂交瘤在脾脏中的培养]

[Cultivation of murine B-cell hybridomas in the spleen].

作者信息

Volgareva G M, Kil'diushevskaia T V, Del'vig A A, Kozhevnikova E V, Sviridov V V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1987 Jan(1):94-8.

Abstract

The tumorigenic capacity of mouse B-cell hybridomas in both cloned and primary cultures was studied. The cells were selected for inoculation from 24-well plates and introduced into the spleen of syngeneic mice. The cells took in 50% of the animals. The cells of hybridoma tumors induced as the result of intrasplenic inoculation, when subcultured in the second passage following the standard scheme, i.e. inoculated intraperitoneally in a dose of 1 X 10(7) cells into mice previously treated with vaseline oil or pristane, produced tumors in 100% of the animals.

摘要

研究了小鼠B细胞杂交瘤在克隆培养和原代培养中的致瘤能力。从24孔板中选择细胞进行接种，并将其引入同基因小鼠的脾脏。这些细胞使50%的动物发生肿瘤。脾内接种诱导产生的杂交瘤肿瘤细胞，按照标准方案在第二代传代培养时，即按1×10⁷个细胞的剂量腹腔接种到预先用凡士林油或 pristane处理过的小鼠体内，100%的动物产生了肿瘤。