Wang Daijie, Khan Muhammad Shafiq, Cui Li, Song Xiangyun, Zhu Heng, Ma Tianyu, Li Xiaoyu, Sun Rong
Key Laboratory of TCM Quality Control, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences) Jinan 250014 P. R. China.
Department of Biotechnology and Bioinfomatics, International Islamic University Islamabad 44000 Islamic Republic of Pakistan.
RSC Adv. 2019 Feb 7;9(9):4892-4899. doi: 10.1039/c8ra10629k. eCollection 2019 Feb 5.
Genistein, an important soybean isoflavone compound, has gained attention for its significant properties. Compared with the glycone form of genistin, low content of genistein limits the use in food and pharmaceutical fields. In this study, a novel bioreactor with high-speed counter-current chromatography (HSCCC) was built for the highly efficient biotransformation of genistein from genistin. The solvent system for the bioreactor was selected according to the values. The selected solvent system was evaluated by the enzyme activity of β-glucosidase. An ethyl acetate/buffer solution was selected as the preferred solvent system for the HSCCC bioreactor. Optimum reactor parameters were selected according to the retention of the stationary phase. The HSCCC bioreactor was operated using different flow rates, and 2.0 mL min was chosen as the optimal flow rate with a conversion rate of over 90% within 24 h; the novel bioreactor easily immobilized and recycled the enzyme and could be applied in the preparation of genistein.
染料木黄酮是一种重要的大豆异黄酮化合物，因其显著特性而受到关注。与染料木苷的糖苷形式相比，染料木黄酮含量较低限制了其在食品和制药领域的应用。在本研究中，构建了一种新型高速逆流色谱（HSCCC）生物反应器，用于从染料木苷高效生物转化生成染料木黄酮。根据 值选择生物反应器的溶剂系统。通过β-葡萄糖苷酶的酶活性评估所选溶剂系统。选择乙酸乙酯/缓冲溶液作为HSCCC生物反应器的首选溶剂系统。根据固定相的保留情况选择最佳反应器参数。使用不同流速操作HSCCC生物反应器，选择2.0 mL/min作为最佳流速，24小时内转化率超过90%；这种新型生物反应器易于固定化和循环利用酶，可应用于染料木黄酮的制备。
