Pharmacognosy Department, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt.
Department of Medical Biochemistry, National Research Center, 33 El Bohouth St. (Former El Tahrir St.), P.O. 12622, Dokki, Giza, Egypt.
Sci Rep. 2024 Aug 12;14(1):18690. doi: 10.1038/s41598-024-68675-2.
Styphnolobium japonicum leaves are considered a rich source of flavonoids, which are the prospective basis for various therapeutic effects. However, there has been a lack of comprehensive cytotoxic studies conducted on these leaves. Therefore, this ongoing investigation aimed to detect and isolate the flavonoids present in S. japonicum leaves, and assess their antioxidant and anticancer properties. The defatted extract from S. japonicum leaves was analyzed using HPLC, which resulted in the identification of seven phenolics and six flavonoids. Rutin and quercetin were found to be the most abundant. Furthermore, a comprehensive profile of flavonoids was obtained through UPLC/ESI-MS analysis in negative acquisition mode. Fragmentation pathways of the identified flavonoids were elucidated to gain relevant insights into their structural characteristics. Furthermore, genistein 7-O-glucoside, quercetin 3-O-rutinoside, and kaempferol 3-O-α-L-rhamnopyranosyl-(1 → 6)-β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranoside were isolated and characterized. The defatted extract rich in flavonoids exhibited significant antioxidant, iron-reducing, free radicals scavenging impacts, and remarkable cytotoxicity against the liver cell line (IC 337.9μg/ mL) and lung cell line (IC 55.0 μg/mL). Furthermore, the antioxidant and anticancer capacities of the three isolated flavonoids have been evaluated, and it has been observed that their effects are concentration-dependent. The findings of this research highlight the promising impact of flavonoids in cancer therapy. It is recommended that future scientific investigations prioritize the exploration of the distinct protective and therapeutic characteristics of S. japonicum leaves, which hold significant potential as a valuable natural resource.
皂荚叶被认为是黄酮类化合物的丰富来源，这些化合物是各种治疗效果的潜在基础。然而，对于这些叶子还缺乏全面的细胞毒性研究。因此，本研究旨在检测和分离皂荚叶中的黄酮类化合物，并评估其抗氧化和抗癌特性。采用高效液相色谱法对皂荚叶脱脂提取物进行分析，结果鉴定出 7 种酚类和 6 种黄酮类化合物。芦丁和槲皮素的含量最高。此外，还通过 UPLC/ESI-MS 分析在负离子采集模式下获得了黄酮类化合物的综合图谱。通过解析鉴定出的黄酮类化合物的裂解途径，深入了解它们的结构特征。此外，还分离并鉴定出染料木素 7-O-葡萄糖苷、槲皮素 3-O-芦丁苷和山奈酚 3-O-α-L-鼠李吡喃糖基-(1 → 6)-β-D-吡喃葡萄糖基-(1 → 2)-β-D-吡喃葡萄糖苷。富含黄酮类化合物的脱脂提取物表现出显著的抗氧化、铁还原、自由基清除作用，对肝癌细胞系（IC 337.9μg/ mL）和肺癌细胞系（IC 55.0μg/mL）具有显著的细胞毒性。此外，还评估了三种分离出的黄酮类化合物的抗氧化和抗癌能力，结果表明它们的作用具有浓度依赖性。本研究结果强调了黄酮类化合物在癌症治疗中的应用前景。建议未来的科学研究优先探索皂荚叶的独特保护和治疗特性，因为皂荚叶作为一种有价值的天然资源具有巨大的潜力。
