Jinbo Daisuke, Imato Keiichi, Ooyama Yousuke
Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University Higashi-Hiroshima 739-8527 Japan
RSC Adv. 2019 May 16;9(27):15335-15340. doi: 10.1039/c9ra02686j. eCollection 2019 May 14.
An anthracene-(aminomethyl)phenylboronic acid ester-BODIPY (DJ-1) was designed and developed as a fluorescent sensor based on photo-induced electron transfer (PET) and Förster resonance energy transfer (FRET) for the detection of a trace amount of water in solvents, where the anthracene skeleton and BODIPY skeleton are the donor fluorophore and the acceptor fluorophore in the FRET process, respectively. It was found that the addition of water to organic solvents containing DJ-1 causes both the suppression of PET in the anthracene-(aminomethyl)phenylboronic acid ester as the PET-type fluorescent sensor skeleton and the energy transfer from the anthracene skeleton to the BODIPY skeleton through a FRET process, thus resulting in the enhancement of the fluorescence band originating from the BODIPY skeleton. This work demonstrates that the PET/FRET-based fluorescent dye composed of the donor fluorophore possessing PET characteristics and the acceptor fluorophore in the FRET process can act as a fluorescent sensor with a large SS for the detection of a trace amount of water in solvents.
设计并开发了一种蒽 -（氨甲基）苯基硼酸酯 - 硼二吡咯（DJ - 1）作为基于光诱导电子转移（PET）和福斯特共振能量转移（FRET）的荧光传感器，用于检测溶剂中的痕量水，其中蒽骨架和硼二吡咯骨架在FRET过程中分别作为供体荧光团和受体荧光团。研究发现，向含有DJ - 1的有机溶剂中加水，既会抑制作为PET型荧光传感器骨架的蒽 -（氨甲基）苯基硼酸酯中的PET，又会通过FRET过程使能量从蒽骨架转移到硼二吡咯骨架，从而导致源自硼二吡咯骨架的荧光带增强。这项工作表明，由具有PET特性的供体荧光团和FRET过程中的受体荧光团组成的基于PET/FRET的荧光染料可作为一种具有大斯托克斯位移（SS）的荧光传感器，用于检测溶剂中的痕量水。
