Wang Lu, Wang Jianbo, Xia Shuai, Wang Xinxin, Yu Yating, Zhou Hongwei, Liu Haiying
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China.
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China.
Talanta. 2020 Nov 1;219:121296. doi: 10.1016/j.talanta.2020.121296. Epub 2020 Jun 27.
A new fluorescent probe A with BODIPY as FRET donor and near-infrared rhodamine as FRET acceptor is constructed through disulfide bonding and use for ratiometric fluorescence detection of biothiol. Due to the efficient fluorescence resonance energy transfer (FRET) from BODIPY donor to near-infrared rhodamine acceptor, Probe A only displays near-infrared rhodamine fluorescence (λ = 656 nm) under BODIPY excitation at 480 nm. The presence of biothiol leads to BODIPY fluorescence increases (λ = 511 nm) and near-infrared rhodamine fluorescence decreases since the disulfide bond of the probe is broken by biothiols, effectively separating the donor from the acceptor, thus inhibiting the FRET process. Probe A exhibits remarkable high selectivity and excellent linear relationship from 10 μM to 100 μM of GSH, with low detection limit as 0.26 μM. Cellular imaging experiments shows that the probe is predominantly present in mitochondria and has been successfully applied to detect biothiol concentrations changes in mitochondria of living cells.
通过二硫键构建了一种以BODIPY为荧光共振能量转移（FRET）供体、近红外罗丹明为FRET受体的新型荧光探针A，并将其用于生物硫醇的比率荧光检测。由于从BODIPY供体到近红外罗丹明受体的高效荧光共振能量转移（FRET），探针A在480 nm的BODIPY激发下仅显示近红外罗丹明荧光（λ = 656 nm）。生物硫醇的存在导致BODIPY荧光增强（λ = 511 nm）且近红外罗丹明荧光减弱，因为探针的二硫键被生物硫醇破坏，有效地将供体与受体分离，从而抑制了FRET过程。探针A对10 μM至100 μM的谷胱甘肽（GSH）表现出显著的高选择性和优异的线性关系，检测限低至0.26 μM。细胞成像实验表明，该探针主要存在于线粒体中，并已成功应用于检测活细胞线粒体中生物硫醇浓度的变化。
