一种生物素引导的硫化氢荧光探针及其在活细胞成像中的应用。
A biotin-guided hydrogen sulfide fluorescent probe and its application in living cell imaging.
作者信息
Zhang Chen, Zhang Jiewen, Xu Zhiqiang, Zang Kun, Liu Feng, Yin Jun, Tan Ying, Jiang Yuyang
机构信息
State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Biology, Tsinghua Shenzhen International Graduate School, Tsinghua University Shenzhen 518055 P. R. China
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University Wuhan 430079 China
出版信息
RSC Adv. 2020 Oct 1;10(59):36135-36140. doi: 10.1039/d0ra06524b. eCollection 2020 Sep 28.
Hydrogen sulfide (HS), a well-known signaling molecule, exerts significant regulatory effects on the cardiovascular and nervous systems. Therefore, monitoring the metabolism of HS offers a potential mechanism to detect various diseases. In addition, biotin is significantly used as a targeting group to detect cancer cells exclusively. In this work, a biotin-guided benzoxadizole-based fluorescent probe, NP-biotin, was developed for HS detection and evaluated in normal liver cell (LO2) and liver cancer cell (HepG2) lines. Results reveal that NP-biotin can detect cellular HS with high sensitivity and selectivity. Moreover, NP-biotin has been confirmed to possess the ability to target cancer cells under the guidance of the biotin group.
硫化氢（HS）是一种著名的信号分子，对心血管和神经系统具有显著的调节作用。因此，监测HS的代谢为检测各种疾病提供了一种潜在机制。此外，生物素被大量用作专门检测癌细胞的靶向基团。在这项工作中，开发了一种生物素引导的基于苯并恶二唑的荧光探针NP-生物素，用于检测HS，并在正常肝细胞系（LO2）和肝癌细胞系（HepG2）中进行了评估。结果表明，NP-生物素能够高灵敏度和高选择性地检测细胞内的HS。此外，已证实NP-生物素在生物素基团的引导下具有靶向癌细胞的能力。
Zotero 插件