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一种生物素引导的硫化氢荧光探针及其在活细胞成像中的应用。

A biotin-guided hydrogen sulfide fluorescent probe and its application in living cell imaging.

作者信息

Zhang Chen, Zhang Jiewen, Xu Zhiqiang, Zang Kun, Liu Feng, Yin Jun, Tan Ying, Jiang Yuyang

机构信息

State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Biology, Tsinghua Shenzhen International Graduate School, Tsinghua University Shenzhen 518055 P. R. China

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University Wuhan 430079 China

出版信息

RSC Adv. 2020 Oct 1;10(59):36135-36140. doi: 10.1039/d0ra06524b. eCollection 2020 Sep 28.

DOI:10.1039/d0ra06524b
PMID:35517112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9056979/
Abstract

Hydrogen sulfide (HS), a well-known signaling molecule, exerts significant regulatory effects on the cardiovascular and nervous systems. Therefore, monitoring the metabolism of HS offers a potential mechanism to detect various diseases. In addition, biotin is significantly used as a targeting group to detect cancer cells exclusively. In this work, a biotin-guided benzoxadizole-based fluorescent probe, NP-biotin, was developed for HS detection and evaluated in normal liver cell (LO2) and liver cancer cell (HepG2) lines. Results reveal that NP-biotin can detect cellular HS with high sensitivity and selectivity. Moreover, NP-biotin has been confirmed to possess the ability to target cancer cells under the guidance of the biotin group.

摘要

硫化氢（HS）是一种著名的信号分子，对心血管和神经系统具有显著的调节作用。因此，监测HS的代谢为检测各种疾病提供了一种潜在机制。此外，生物素被大量用作专门检测癌细胞的靶向基团。在这项工作中，开发了一种生物素引导的基于苯并恶二唑的荧光探针NP-生物素，用于检测HS，并在正常肝细胞系（LO2）和肝癌细胞系（HepG2）中进行了评估。结果表明，NP-生物素能够高灵敏度和高选择性地检测细胞内的HS。此外，已证实NP-生物素在生物素基团的引导下具有靶向癌细胞的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/e31e63d9829a/d0ra06524b-f10.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/e6ad4654d0f9/d0ra06524b-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/e31e63d9829a/d0ra06524b-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/f9b8d4744340/d0ra06524b-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/45f29e7507c5/d0ra06524b-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/08ea0bd06fe1/d0ra06524b-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/5a8dccc646c7/d0ra06524b-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/e31e9d5b8e59/d0ra06524b-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/ea8c229b3ff0/d0ra06524b-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/22f1d8f2d738/d0ra06524b-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/f7eac5b62d31/d0ra06524b-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/e6ad4654d0f9/d0ra06524b-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fa9/9056979/e31e63d9829a/d0ra06524b-f10.jpg

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本文引用的文献

1
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J Mater Chem B. 2017 Feb 7;5(5):988-995. doi: 10.1039/c6tb02957d. Epub 2017 Jan 17.
2
Biotinylated amphiphile-single walled carbon nanotube conjugate for target-specific delivery to cancer cells.用于靶向特异性递送至癌细胞的生物素化两亲物-单壁碳纳米管共轭物。
J Mater Chem B. 2014 Mar 7;2(9):1160-1173. doi: 10.1039/c3tb21334j. Epub 2014 Jan 20.
3
Fluorescent Probes for the Visualization of Cell Viability.
用于细胞活力可视化的荧光探针。
Acc Chem Res. 2019 Aug 20;52(8):2147-2157. doi: 10.1021/acs.accounts.9b00289. Epub 2019 Jul 23.
4
Coumarin-Based Small-Molecule Fluorescent Chemosensors.香豆素基小分子荧光化学传感器。
Chem Rev. 2019 Sep 25;119(18):10403-10519. doi: 10.1021/acs.chemrev.9b00145. Epub 2019 Jul 17.
5
Strategies for designing organic fluorescent probes for biological imaging of reactive carbonyl species.用于活性羰基物种生物成像的有机荧光探针设计策略。
Chem Soc Rev. 2019 Jul 29;48(15):4036-4048. doi: 10.1039/c8cs00956b.
6
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Adv Sci (Weinh). 2018 May 1;5(7):1700966. doi: 10.1002/advs.201700966. eCollection 2018 Jul.
7
Gasotransmitter hydrogen sulfide signaling in neuronal health and disease.气体递质硫化氢在神经元健康和疾病中的信号转导作用。
Biochem Pharmacol. 2018 Mar;149:101-109. doi: 10.1016/j.bcp.2017.11.019. Epub 2017 Dec 1.
8
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Chem Sci. 2017 Sep 1;8(9):6257-6265. doi: 10.1039/c7sc00423k. Epub 2017 Jun 30.
9
Conjugate of biotin with silicon(IV) phthalocyanine for tumor-targeting photodynamic therapy.生物素与硅(IV)酞菁的缀合物用于肿瘤靶向光动力治疗。
J Photochem Photobiol B. 2017 Sep;174:243-250. doi: 10.1016/j.jphotobiol.2017.08.003. Epub 2017 Aug 3.
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Talanta. 2017 Nov 1;174:357-364. doi: 10.1016/j.talanta.2017.06.023. Epub 2017 Jun 13.