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一种用于高效树脂纯化在哺乳动物系统中表达的His标签蛋白的多羧酸螯合配体。

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems.

作者信息

Popescu Codruţa C, Stoian Marius C, Cucos Lia-Maria, Coman Anca G, Radoi Antonio, Paun Anca, Hădade Niculina D, Gautier Arnaud, Popescu Costin-Ioan, Matache Mihaela

机构信息

University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Research Centre of Applied Organic Chemistry 90-92 Panduri Street RO-050663 Bucharest Romania

National Institute for Research and Development in Microtechnology - IMT Bucharest 126A Erou Iancu Nicolae Street 077190 Voluntari Romania.

出版信息

RSC Adv. 2020 Jun 23;10(40):23931-23935. doi: 10.1039/d0ra02382e. eCollection 2020 Jun 19.

DOI:10.1039/d0ra02382e
PMID:35517349
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9055129/
Abstract

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.

摘要

我们描述了一种新型多氨基多羧酸配体的合成、其与金属离子配位的能力以及在通过固定化金属离子亲和色谱法（IMAC）进行蛋白质纯化的固体支持物上的附着情况。结果发现该树脂对于纯化在293T哺乳动物细胞中表达的His标签丙型肝炎病毒E2糖蛋白非常高效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/4299481a1e42/d0ra02382e-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/f84d20f44327/d0ra02382e-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/e2a18d6c24c0/d0ra02382e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/d490b14b5c1f/d0ra02382e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/19e116f5417b/d0ra02382e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/d5b48677af3b/d0ra02382e-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/3ff74f780b6a/d0ra02382e-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/4299481a1e42/d0ra02382e-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/f84d20f44327/d0ra02382e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/db78680b5474/d0ra02382e-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/e2a18d6c24c0/d0ra02382e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/d490b14b5c1f/d0ra02382e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/19e116f5417b/d0ra02382e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/d5b48677af3b/d0ra02382e-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/3ff74f780b6a/d0ra02382e-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1fe/9055129/4299481a1e42/d0ra02382e-f7.jpg

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