Liang Jing, Yu Lei, Lin Ziying, Song Keji, Zhang Jiejing, Zhang Jianfeng
College of Life Science, Jilin Agricultural University, Jilin Province Innovation Platform of Straw Comprehensive Utilization Technology Changchun 130118 China
Jilin Radio and TV University Changchun 130022 China.
RSC Adv. 2019 Jan 4;9(2):699-703. doi: 10.1039/c8ra09662g. eCollection 2019 Jan 2.
A simple and cheap method to fabricate a nanocrystal ring pattern was developed by utilization of a microemulsion in this study. The mixture of polystyrene and stabilizer dichloromethane solution that contained nanocrystal aqueous solution, prepared through shaking, was applied to fabricate a reverse microemulsion. After spreading and evaporating the solvent of microemulsion on a glass slide, an ordered honeycomb film was produced, accompanied by the formation of a nanocrystal ring pattern. The nanocrystal pattern could be readily applied for immunoassays and recognition of proteins. The pattern with antibody marked by a green colored nanocrystal specifically bound with antigen labeled by a red colored nanocrystal, leading to the enhancement in red fluorescent ring pattern and decrease in green fluorescent pattern. When the unlabeled antigen was added, the green fluorescent pattern was recovered. In addition, the ring pattern with immunocomplex could selectively recognize antigen and transferrin proteins. This strategy reveals that these patterns have potential applications in biochips, biosensors, imaging analysis and so forth.
在本研究中，通过利用微乳液开发了一种简单且廉价的制备纳米晶体环形图案的方法。将含有纳米晶体水溶液的聚苯乙烯和稳定剂二氯甲烷溶液通过振荡混合，用于制备反相微乳液。在玻璃载玻片上铺展并蒸发微乳液的溶剂后，形成了有序的蜂窝状薄膜，并伴随着纳米晶体环形图案的形成。该纳米晶体图案可很容易地应用于免疫测定和蛋白质识别。用绿色纳米晶体标记的抗体图案与用红色纳米晶体标记的抗原特异性结合，导致红色荧光环形图案增强，绿色荧光图案减弱。当加入未标记的抗原时，绿色荧光图案恢复。此外，带有免疫复合物的环形图案可以选择性地识别抗原和转铁蛋白。该策略表明，这些图案在生物芯片、生物传感器、成像分析等方面具有潜在应用。
