Liang Jing, Yu Lei, Li Xue, Zhang Jiejing, Chen Guang, Zhang Jianfeng
College of Life Science, Jilin Agricultural University, Key Laboratory of Straw Biology and Utilization, The Ministry of Education Changchun 130118 China
Jilin Radio and TV University Changchun 130022 China.
RSC Adv. 2019 Aug 28;9(46):26838-26842. doi: 10.1039/c9ra05719f. eCollection 2019 Aug 23.
Quantum dot microstructures were fabricated through a convenient microemulsion approach in this study. A polymer solution containing a stabilizer was mixed with a quantum dot aqueous solution, to prepare a reversed microemulsion, through shaking. Then, the microemulsion was cast on a solid substrate followed by evaporating steps, resulting in the formation of an ordered porous film. Interestingly, the quantum dot microstructure can be produced at the same time. The immunoassay experiment could be realized by the fluorescent microstructures. The green fluorescence microstructure specifically bound with antigens marked with red color quantum dots, resulting in the enhancement of red fluorescence domains and the decrease of green fluorescence. With the addition of unlabeled antigens, the green fluorescence microstructure was recovered. This strategy implies that the quantum dot pattern has potential on biochip, biosensor, and imaging analysis.
在本研究中，通过一种简便的微乳液方法制备了量子点微结构。将含有稳定剂的聚合物溶液与量子点水溶液混合，通过振荡制备反相微乳液。然后，将微乳液浇铸在固体基质上，随后进行蒸发步骤，从而形成有序的多孔膜。有趣的是，同时可以产生量子点微结构。免疫分析实验可以通过荧光微结构来实现。绿色荧光微结构与用红色量子点标记的抗原特异性结合，导致红色荧光区域增强而绿色荧光减弱。加入未标记的抗原后，绿色荧光微结构得以恢复。该策略表明量子点图案在生物芯片、生物传感器和成像分析方面具有潜力。
