Zhu Xiaomei, Ji WenQing, Guo Shubin, Zhu Di, Yang Yue, Liu Xin
Beijing Key Laboratory of Cardiopulmonary-Cerebral Resuscitation, Emergency Department, Chao-Yang Hospital, Capital Medical University Chaoyang District Beijing 100020 China
RSC Adv. 2020 Jun 3;10(35):21000-21008. doi: 10.1039/d0ra01947j. eCollection 2020 May 27.
Immunometabolism determines the fate and function of regulatory T cells. The metabolic phenotype of regulatory T cells (Treg) is affected by various factors. The relationship between Treg metabolism and function of mice with sepsis is not clear. We used liquid chromatography and tandem mass spectrometry (LC-MS/MS) to analyze the metabolic profiles of freshly-isolated spleen Treg cells in mice with sepsis. It was found that in severe infection, activated Treg cells depend on glycolysis and fatty acid oxidation, and inhibition of metabolic pathways has a significant impact on the number and quality of Treg cells. Understanding the metabolic characteristics of Treg cells in the real environment in the body helps to grasp the function of Treg cells and even the overall immune status. Targeting the metabolic pathway of Treg may provide a new method for the treatment of sepsis.
免疫代谢决定调节性T细胞的命运和功能。调节性T细胞（Treg）的代谢表型受多种因素影响。脓毒症小鼠中Treg代谢与功能之间的关系尚不清楚。我们使用液相色谱和串联质谱（LC-MS/MS）分析脓毒症小鼠新鲜分离的脾脏Treg细胞的代谢谱。研究发现，在严重感染时，活化的Treg细胞依赖糖酵解和脂肪酸氧化，抑制代谢途径对Treg细胞的数量和质量有显著影响。了解体内真实环境中Treg细胞的代谢特征有助于掌握Treg细胞的功能乃至整体免疫状态。针对Treg的代谢途径可能为脓毒症的治疗提供一种新方法。
