Shen Luhui, Zhao Zhan-Gong, Lainson John C, Brown Justin R, Sykes Kathryn F, Johnston Stephen Albert, Diehnelt Chris W
Center for Innovations in Medicine, Biodesign Institute, Arizona State University Tempe AZ USA
Calviri, Inc. Tempe AZ USA.
RSC Adv. 2020 Aug 11;10(50):29675-29681. doi: 10.1039/d0ra05267a. eCollection 2020 Aug 10.
Parallel measurement of large numbers of antigen-antibody interactions are increasingly enabled by peptide microarray technologies. Our group has developed an synthesized peptide microarray of >400 000 frameshift neoantigens using mask-based photolithographic peptide synthesis, to profile patient specific neoantigen reactive antibodies in a single assay. The system produces 208 replicate mircoarrays per wafer and is capable of producing multiple wafers per synthetic lot to routinely synthesize over 300 million peptides simultaneously. In this report, we demonstrate the feasibility of the system for detecting peripheral-blood antibody binding to frameshift neoantigens across multiple synthetic lots.
肽微阵列技术越来越能够实现大量抗原-抗体相互作用的并行测量。我们的团队利用基于掩膜的光刻肽合成技术，开发了一种包含超过40万个移码新抗原的合成肽微阵列，以便在一次检测中分析患者特异性新抗原反应性抗体。该系统每个晶片可生产208个重复微阵列，并且每个合成批次能够生产多个晶片，从而能够同时常规合成超过3亿个肽。在本报告中，我们证明了该系统在多个合成批次中检测外周血抗体与移码新抗原结合的可行性。
