Li Youdong, Li Jinwei, Cao Peirang, Liu Yuanfa
School of Food Science and Technology, Jiangnan University 1800 Lihu Road Wuxi 214122 Jiangsu People's Republic of China
State Key Laboratory of Food Science and Technology, National Engineering Laboratory for Cereal Fermentation Technology, National Engineering Research Centre for Functional Food, Jiangnan University 1800 Lihu Road Wuxi 214122 Jiangsu People's Republic of China.
RSC Adv. 2020 Jun 3;10(36):21248-21258. doi: 10.1039/d0ra00215a. eCollection 2020 Jun 2.
Oil enrichment with trace amounts of components has significant effects on animal nutrition and health. In this work, the potential impact of sinapine, a trace amount of polyphenol naturally present in rapeseeds, was investigated in high-fat diet (HF)-fed C57BL/6J mice. The mice were fed with different diets including chow diet (LF), HF diet, rapeseed oil-containing HF diet (RO), and rapeseed oils enriched with sinapine (500 mg kg oil, high-fat diet, RP) for 12 weeks. Here, it was demonstrated that sinapine supplementation significantly reduced ( < 0.05) body weight increase, fat accumulation, and fatty liver formation in mice when compared with those fed with a high-fat diet. The TG, LDL-C, ALT and AST levels in the RP group were significantly reduced ( < 0.05) by 15.67%, 73.62%, 20.67%, and 31.58%, respectively, compared with that in the HF group. Besides, the addition of sinapine prevented the degeneration of mouse adipocytes and lipid accumulation in the liver. Moreover, this change was achieved by downregulating SREBP-1c and FAS and upregulating PPAR-α and ACOX1 gene expression levels. Our results indicate that sinapine can be used as a prebiotic to enhance the nutritional function of vegetable oils to prevent obesity-related chronic diseases such as NAFLD.
富含痕量成分的油脂对动物营养和健康有显著影响。在本研究中，我们研究了油菜籽中天然存在的痕量多酚——芥子碱对高脂饮食（HF）喂养的C57BL/6J小鼠的潜在影响。将小鼠分别喂食不同的饮食，包括普通饮食（LF）、高脂饮食、含菜籽油的高脂饮食（RO）和富含芥子碱的菜籽油（500 mg/kg油，高脂饮食，RP），持续12周。结果表明，与高脂饮食喂养的小鼠相比，补充芥子碱可显著降低（<0.05）小鼠的体重增加、脂肪堆积和脂肪肝形成。与HF组相比，RP组的TG、LDL-C、ALT和AST水平分别显著降低（<0.05）15.67%、73.62%、20.67%和31.58%。此外，添加芥子碱可防止小鼠脂肪细胞退化和肝脏脂质堆积。而且，这种变化是通过下调SREBP-1c和FAS以及上调PPAR-α和ACOX1基因表达水平实现的。我们的结果表明，芥子碱可作为益生元来增强植物油的营养功能，预防肥胖相关的慢性疾病，如非酒精性脂肪性肝病。
