College of Medicine, Southwest Jiaotong University, Chengdu, China.
Department of Orthopedics, General Hospital of Western Theater Command, Chengdu, China.
Front Endocrinol (Lausanne). 2022 Apr 19;13:849076. doi: 10.3389/fendo.2022.849076. eCollection 2022.
As an important public health problem, osteoporosis (OP) in China is also in an upward trend year by year. As a standard method for diagnosing OP, dual-energy X-ray absorptiometry (DXA) cannot analyze the pathological process but only see the results. It is difficult to evaluate the early diagnosis of OP. Our study was carried out through a serum metabolomic study of OP in Chinese postmenopausal women on untargeted gas chromatography (GC)/liquid chromatography (LC)-mass spectrometry (MS) to find possible diagnostic markers.
50 Chinese postmenopausal women with osteoporosis and 50 age-matched women were selected as normal controls. We first used untargeted GC/LC-MS to analyze the serum of these participants and then combined it with a large number of multivariate statistical analyses to analyze the data. Finally, based on a multidimensional analysis of the metabolites, the most critical metabolites were considered to be biomarkers of OP in postmenopausal women. Further, biomarkers identified relevant metabolic pathways, followed by a map of metabolic pathways found in the database.
We found that there may be metabolic pathway disorders like glucose metabolism, lipid metabolism, and amino acid metabolism in postmenopausal women with OP. 18 differential metabolites are considered to be potential biomarkers of OP in postmenopausal women which are a major factor in metabolism and bone physiological function.
These findings can be applied to clinical work through further validation studies. It also shows that metabonomic analysis has great potential in the application of early diagnosis and recurrence monitoring in postmenopausal OP women.
骨质疏松症（OP）作为一个重要的公共卫生问题，在中国也呈逐年上升趋势。双能 X 射线吸收法（DXA）作为诊断 OP 的标准方法，不能分析其病理过程，只能看到结果，难以评估 OP 的早期诊断。本研究通过对中国绝经后妇女进行 OP 的血清代谢组学研究，采用非靶向气相色谱（GC）/液相色谱（LC）-质谱（MS），寻找可能的诊断标志物。
选择 50 例骨质疏松症绝经后妇女和 50 例年龄匹配的正常妇女作为正常对照组。我们首先采用非靶向 GC/LC-MS 分析这些参与者的血清，然后结合大量多元统计分析对数据进行分析。最后，基于对代谢物的多维分析，认为最关键的代谢物是绝经后妇女 OP 的生物标志物。进一步鉴定生物标志物相关代谢途径，然后在数据库中找到代谢途径图。
我们发现，OP 绝经后妇女可能存在葡萄糖代谢、脂质代谢和氨基酸代谢等代谢途径紊乱。18 种差异代谢物被认为是绝经后妇女 OP 的潜在生物标志物，这些标志物是代谢和骨骼生理功能的重要因素。
这些发现可以通过进一步的验证研究应用于临床工作。这也表明代谢组学分析在绝经后 OP 妇女的早期诊断和复发监测的应用中具有很大的潜力。
