College of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, People's Republic of China.
Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA.
Menopause. 2019 Jan;26(1):94-102. doi: 10.1097/GME.0000000000001158.
In the present study, we aimed to characterize the pathological development of menopausal osteoporosis, as well as to explore potential biomarkers and metabolic pathways involved in osteoporosis.
Urine samples from 322 female participants categorized by menopause status and different bone conditions were collected and analyzed based on a gas chromatography-mass spectrometry (GC-MS) approach. Multivariate and univariate statistical analyses were carried out for urinary metabolomic profile characterization and comparison.
Seventeen metabolites in the low bone mineral density (BMD) groups were clearly differentiated from those in normal BMD groups. Among these 17 differentiating metabolites, taurine, β-alanine, and 5-hydroxycaproic acid were found to be potential biomarkers of osteoporosis. The taurine metabolic pathway and the β-alanine metabolic pathway were found to be related to menopause and bone loss.
Based on the GC-MS metabolomic platform, four typical pathological phases during the progression of postmenopausal osteoporosis were described. Several differentiating metabolites and metabolic pathways were found to be closely related to the pathology of postmenopausal osteoporosis. Our results provided a solid foundation for further studies on early diagnosis and pathomechanistic evaluation.
本研究旨在描述绝经后骨质疏松症的病理发展，并探讨骨质疏松症相关的潜在生物标志物和代谢途径。
根据气相色谱-质谱（GC-MS）分析方法，收集并分析了 322 名按绝经状态和不同骨骼状况分类的女性参与者的尿样。对尿代谢组特征和比较进行了多变量和单变量统计分析。
17 种在低骨密度（BMD）组中明显与正常 BMD 组不同的代谢物被区分出来。在这 17 种有区别的代谢物中，牛磺酸、β-丙氨酸和 5-羟基己酸被认为是骨质疏松症的潜在生物标志物。牛磺酸代谢途径和β-丙氨酸代谢途径与绝经和骨质流失有关。
基于 GC-MS 代谢组学平台，描述了绝经后骨质疏松症进展过程中的四个典型病理阶段。发现一些有区别的代谢物和代谢途径与绝经后骨质疏松症的病理密切相关。我们的结果为进一步进行早期诊断和病理机制评估的研究提供了坚实的基础。
