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一种基于CRISPR-Cas9修饰的催化发夹组装的用于微小RNA检测和成像的灵敏且特异的方法。

A sensitive and specific method for microRNA detection and imaging based on a CRISPR-Cas9 modified catalytic hairpin assembly.

作者信息

Liu Yang, Li Shihong, Zhang Likun, Zhao Qian, Li Nuo, Wu Yuxin

机构信息

Department of Clinical Laboratory, The Third Affiliated Hospital of Jinzhou Medical University Jinzhou Liaoning Province 121000 China

出版信息

RSC Adv. 2020 Jul 28;10(47):28037-28040. doi: 10.1039/d0ra03603j. eCollection 2020 Jul 27.

DOI:10.1039/d0ra03603j
PMID:35519131
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9055656/
Abstract

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR-Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR-Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection and miR-21 imaging.

摘要

我们在此报告一种基于CRISPR-Cas9和催化发夹组装的外泌体中微小RNA（miRNA）分子检测及活细胞成像方法。凭借催化发夹组装的级联信号放大和CRISPR-Cas9的高特异性识别，该检测方法灵敏度达23.5飞摩尔，可用于准确检测外泌体miR-21及对miR-21进行成像。

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