Wen Chumao, Li Jun, Xu Xiaoshi, Li Qingqing, Li Yitong, Chen Yu, Yang Guangzhong
College of Biomedical Engineering, South-Central University for Nationalities Wuhan 430074 P. R. China.
School of Pharmaceutical Sciences, South-Central University for Nationalities Wuhan 430074 P. R. China
RSC Adv. 2020 Jul 28;10(47):28157-28163. doi: 10.1039/d0ra04917d. eCollection 2020 Jul 27.
Sixteen lignans with diverse carbon skeletons, including tetrahydrofuran lignans (1, 13-18), 7,9'-dinorlignan (2), dibenzylbutane lignans (9-10), aryl tetrahydronaphthalene lignans (11-12), dihydrobenzofuran neolignans (19-20), 8--4' neolignans (21-22), and six clerodane diterpenoid glucosides (3-8) were isolated from the 70% ethanolic extract of the stems of (Lour.) Merr. Their structures were established by spectroscopic analyses and chemical methods. Among all these isolates, compounds 1-2 and 3-4 were determined as previously undescribed lignans and clerodane diterpenoids respectively, and compounds 9-13 and 17-22 were discovered from for the first time. In addition, it was the first time to report three types of lignans including 7,9'-dinorlignan, dihydrobenzofuran neolignan and 8--4' neolignan from . The biological activities of all isolated compounds were assessed in terms of their nitric oxide production inhibitory activity in LPS stimulated RAW264.7 cells. The results showed that compound 20 exhibited a mild NO production inhibitory effect with an IC value of 17.43 ± 2.06 μM.
从（Lour.）Merr.茎的70%乙醇提取物中分离出16种具有不同碳骨架的木脂素，包括四氢呋喃木脂素（1, 13 - 18）、7,9'-二降木脂素（2）、二苄基丁烷木脂素（9 - 10）、芳基四氢萘木脂素（11 - 12）、二氢苯并呋喃新木脂素（19 - 20）、8 - 4'新木脂素（21 - 22），以及6种克罗烷二萜糖苷（3 - 8）。通过光谱分析和化学方法确定了它们的结构。在所有这些分离物中，化合物1 - 2和3 - 4分别被确定为先前未描述的木脂素和克罗烷二萜，化合物9 - 13和17 - 22首次从（该植物）中发现。此外，首次从（该植物）中报道了三种类型的木脂素，包括7,9'-二降木脂素、二氢苯并呋喃新木脂素和8 - 4'新木脂素。根据所有分离化合物在脂多糖刺激的RAW264.7细胞中抑制一氧化氮产生的活性评估了它们的生物活性。结果表明，化合物20表现出温和的一氧化氮产生抑制作用，IC值为17.43 ± 2.06 μM。
