Jiang Ching-Fen, Sun Yu-Man
Graduate Degree Program of Smart Healthcare & Bioinformatics, I-Shou University, Kaohsiung, Taiwan.
Department of Biomedical Engineering, I-Shou University, Kaohsiung, Taiwan.
Biomed Opt Express. 2022 Mar 22;13(4):2323-2333. doi: 10.1364/BOE.452822. eCollection 2022 Apr 1.
Investigation of the dynamic structural changes in the actin cytoskeleton during cell migration provides crucial information about the physiological conditions of a stem cell during in-vitro culture. Here we proposed a quantitative analytical model associated with texture extraction with cell tracking techniques for in situ monitoring of the cytoskeletal density change of stem cells in phase-contrast microscopy without fluorescence staining. The reliability of the model in quantifying the texture density with different orientation was first validated using a series of simulated textural images. The capability of the method to reflect the spatiotemporal regulation of the cytoskeletal structure of a living stem cell was further proved by applying it to a set of 72 h phase-contrast microscopic video of the growth dynamics of mesenchymal stem cells in vitro culture.
研究细胞迁移过程中肌动蛋白细胞骨架的动态结构变化，可为体外培养干细胞的生理状况提供关键信息。在此，我们提出了一种与纹理提取相关的定量分析模型，并结合细胞追踪技术，用于在相差显微镜下原位监测干细胞的细胞骨架密度变化，且无需荧光染色。该模型在量化不同方向纹理密度方面的可靠性首先通过一系列模拟纹理图像得到验证。通过将该方法应用于一组体外培养的间充质干细胞生长动态的72小时相差显微镜视频，进一步证明了该方法反映活干细胞细胞骨架结构时空调控的能力。
