肌动蛋白细胞骨架的超微结构。
Ultrastructure of the actin cytoskeleton.
机构信息
Department of Biology, University of Pennsylvania, 433 S. University Avenue, Philadelphia, PA 19104, USA.
出版信息
Curr Opin Cell Biol. 2018 Oct;54:1-8. doi: 10.1016/j.ceb.2018.02.007. Epub 2018 Feb 21.
Abstract
The actin cytoskeleton is the primary force-generating machinery in the cell, which can produce pushing (protrusive) forces using energy of actin polymerization and pulling (contractile) forces via sliding of bipolar filaments of myosin II along actin filaments, as well as perform other key functions. These functions are essential for whole cell migration, cell interaction with the environment, mechanical properties of the cell surface and other key aspects of cell physiology. The actin cytoskeleton is a highly complex and dynamic system of actin filaments organized into various superstructures by multiple accessory proteins. High resolution architecture of functionally distinct actin arrays provides key clues for understanding actin cytoskeleton functions. This review summarizes recent advance in our understanding of the actin cytoskeleton ultrastructure.
摘要
肌动蛋白细胞骨架是细胞中的主要力产生机制，它可以利用肌动蛋白聚合的能量产生推（突起）力，通过肌球蛋白 II 的双极丝沿着肌动蛋白丝的滑动产生拉（收缩）力，以及执行其他关键功能。这些功能对于整个细胞迁移、细胞与环境的相互作用、细胞表面的机械特性和细胞生理学的其他关键方面都是必不可少的。肌动蛋白细胞骨架是一个高度复杂和动态的肌动蛋白丝系统，由多种辅助蛋白组成各种超结构。具有不同功能的肌动蛋白阵列的高分辨率结构为理解肌动蛋白细胞骨架功能提供了关键线索。这篇综述总结了我们对肌动蛋白细胞骨架超微结构理解的最新进展。
相似文献
1
Ultrastructure of the actin cytoskeleton.肌动蛋白细胞骨架的超微结构。
Curr Opin Cell Biol. 2018 Oct;54:1-8. doi: 10.1016/j.ceb.2018.02.007. Epub 2018 Feb 21.
2
Ultrastructure of protrusive actin filament arrays.伸出的肌动蛋白丝阵列的超微结构。
Curr Opin Cell Biol. 2013 Oct;25(5):574-81. doi: 10.1016/j.ceb.2013.04.003. Epub 2013 Apr 29.
3
The Actin Cytoskeleton and Actin-Based Motility.肌动蛋白细胞骨架和基于肌动蛋白的运动。
Cold Spring Harb Perspect Biol. 2018 Jan 2;10(1):a018267. doi: 10.1101/cshperspect.a018267.
4
The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis.收缩环中肌动蛋白和肌球蛋白II丝的超微结构组织：对胞质分裂旧模型的新支持。
Mol Biol Cell. 2017 Mar 1;28(5):613-623. doi: 10.1091/mbc.E16-06-0466. Epub 2017 Jan 5.
5
A molecular pathway for myosin II recruitment to stress fibers.肌球蛋白 II 向应激纤维募集的分子途径。
Curr Biol. 2011 Apr 12;21(7):539-50. doi: 10.1016/j.cub.2011.03.007. Epub 2011 Mar 31.
6
Crosstalk between myosin II and formin functions in the regulation of force generation and actomyosin dynamics in stress fibers.肌球蛋白II与formin功能之间的相互作用在应力纤维中力的产生和肌动球蛋白动力学的调节中发挥作用。
Cells Dev. 2021 Dec;168:203736. doi: 10.1016/j.cdev.2021.203736. Epub 2021 Aug 26.
7
Dynamics of actin filaments during tension-dependent formation of actin bundles.肌动蛋白丝在张力依赖性肌动蛋白束形成过程中的动力学
Biochim Biophys Acta. 2007 Aug;1770(8):1115-27. doi: 10.1016/j.bbagen.2007.03.010. Epub 2007 Mar 31.
8
Branched actin networks push against each other at adherens junctions to maintain cell-cell adhesion.分支状肌动蛋白网络在黏着连接点相互推挤，以维持细胞间的黏附。
J Cell Biol. 2018 May 7;217(5):1827-1845. doi: 10.1083/jcb.201708103. Epub 2018 Mar 5.
9
Protein localization by actin treadmilling and molecular motors regulates stereocilia shape and treadmilling rate.通过肌动蛋白踏车运动和分子马达进行的蛋白质定位调节静纤毛的形状和踏车运动速率。
Biophys J. 2008 Dec 15;95(12):5706-18. doi: 10.1529/biophysj.108.143453. Epub 2008 Oct 20.
10
Amplification of actin polymerization forces.肌动蛋白聚合力的放大
J Cell Biol. 2016 Mar 28;212(7):763-6. doi: 10.1083/jcb.201512019. Epub 2016 Mar 21.
引用本文的文献
1
MCU-Mediated SIRT3 Inhibition Drives Oxidative Stress-Induced Cytoskeletal Disruption in Pancreatic Ductal Epithelium: A Mechanism of Hypertriglyceridemic Acute Pancreatitis.MCU介导的SIRT3抑制驱动氧化应激诱导的胰腺导管上皮细胞骨架破坏：高甘油三酯血症性急性胰腺炎的一种机制
Int J Gen Med. 2025 Sep 4;18:5195-5211. doi: 10.2147/IJGM.S542782. eCollection 2025.
2
Balancing between cuproplasia and copper-dependent cell death: molecular basis and clinical implications of ATOX1 in cancer.铜代谢异常与铜依赖性细胞死亡之间的平衡：ATOX1在癌症中的分子基础及临床意义
J Exp Clin Cancer Res. 2025 Jul 28;44(1):222. doi: 10.1186/s13046-025-03486-5.
3
Dysfunction of PDE4DIP contributes to LVNC development by regulating cell polarity, skeleton, and energy metabolism via Rho-ROCK pathway.磷酸二酯酶4D相互作用蛋白功能障碍通过Rho-ROCK途径调节细胞极性、骨架和能量代谢，从而导致左心室心肌致密化不全的发展。
Genes Dis. 2025 Feb 21;12(5):101568. doi: 10.1016/j.gendis.2025.101568. eCollection 2025 Sep.
4
Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin.肌动蛋白丝的尖端：原肌球蛋白和原肌球蛋白调节组装与拆卸的检测方法
Cytoskeleton (Hoboken). 2025 Feb 24. doi: 10.1002/cm.22007.
5
Actin dynamics switches two distinct modes of endosomal fusion in yolk sac visceral endoderm cells.肌动蛋白动力学在卵黄囊内脏内胚层细胞中切换两种不同的内体融合模式。
Elife. 2024 Oct 23;13:RP95999. doi: 10.7554/eLife.95999.
6
GALNT9 enrichment attenuates MPP-induced cytotoxicity by ameliorating protein aggregations containing α-synuclein and mitochondrial dysfunction.GALNT9 富集通过改善含有 α-突触核蛋白的蛋白聚集和线粒体功能障碍来减轻 MPP+诱导的细胞毒性。
Biol Direct. 2024 Sep 5;19(1):77. doi: 10.1186/s13062-024-00524-8.
7
Actin filament dynamics at barbed ends: New structures, new insights.肌动蛋白丝在突出端的动力学：新结构，新见解。
Curr Opin Cell Biol. 2024 Oct;90:102419. doi: 10.1016/j.ceb.2024.102419. Epub 2024 Aug 22.
8
Transcriptome and single-cell analysis reveal disulfidptosis-related modification patterns of tumor microenvironment and prognosis in osteosarcoma.转录组和单细胞分析揭示骨肉瘤肿瘤微环境中与二硫键介导的铁死亡相关的修饰模式及预后情况。
Sci Rep. 2024 Apr 22;14(1):9186. doi: 10.1038/s41598-024-59243-9.
9
Benefits and challenges of reconstituting the actin cortex.重构肌动蛋白皮层的益处与挑战。
Cytoskeleton (Hoboken). 2024 Dec;81(12):843-863. doi: 10.1002/cm.21855. Epub 2024 Mar 23.
10
Proteomic analysis reveals the aging-related pathways contribute to pulmonary fibrogenesis.蛋白质组学分析揭示了与衰老相关的途径有助于肺纤维化的发生。
Aging (Albany NY). 2023 Dec 22;15(24):15382-15401. doi: 10.18632/aging.205355.
本文引用的文献
1
The Actin Cytoskeleton and Actin-Based Motility.肌动蛋白细胞骨架和基于肌动蛋白的运动。
Cold Spring Harb Perspect Biol. 2018 Jan 2;10(1):a018267. doi: 10.1101/cshperspect.a018267.
2
Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments.脊椎动物细胞的胞质分裂起始于由随机定向的丝组成的赤道肌动球蛋白网络的收缩。
Elife. 2017 Nov 6;6:e30867. doi: 10.7554/eLife.30867.
3
Load Adaptation of Lamellipodial Actin Networks.片状伪足肌动蛋白网络的负载适应
Cell. 2017 Sep 21;171(1):188-200.e16. doi: 10.1016/j.cell.2017.07.051. Epub 2017 Aug 31.
4
Membrane tension controls adhesion positioning at the leading edge of cells.膜张力控制细胞前缘的黏附定位。
J Cell Biol. 2017 Sep 4;216(9):2959-2977. doi: 10.1083/jcb.201611117. Epub 2017 Jul 7.
5
Actin cortex architecture regulates cell surface tension.肌动蛋白皮层结构调节细胞表面张力。
Nat Cell Biol. 2017 Jun;19(6):689-697. doi: 10.1038/ncb3525. Epub 2017 May 22.
6
Efficiency of lamellipodia protrusion is determined by the extent of cytosolic actin assembly.片状伪足突出的效率取决于胞质肌动蛋白组装的程度。
Mol Biol Cell. 2017 May 15;28(10):1311-1325. doi: 10.1091/mbc.E16-05-0334. Epub 2017 Mar 22.
7
FMNL formins boost lamellipodial force generation.FMNL 形成蛋白促进片状伪足的力生成。
Nat Commun. 2017 Mar 22;8:14832. doi: 10.1038/ncomms14832.
8
Platinum replica electron microscopy: Imaging the cytoskeleton globally and locally.铂复型电子显微镜术：对细胞骨架进行整体和局部成像。
Int J Biochem Cell Biol. 2017 May;86:37-41. doi: 10.1016/j.biocel.2017.03.009. Epub 2017 Mar 18.
9
Differential role of nonmuscle myosin II isoforms during blebbing of MCF-7 cells.非肌肉肌球蛋白II亚型在MCF-7细胞气泡形成过程中的差异作用。
Mol Biol Cell. 2017 Apr 15;28(8):1034-1042. doi: 10.1091/mbc.E16-07-0524. Epub 2017 Mar 1.
10
Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.肌动蛋白动力学和对肌球蛋白单体的竞争决定了肌球蛋白丝的顺序放大。
Nat Cell Biol. 2017 Feb;19(2):85-93. doi: 10.1038/ncb3463. Epub 2017 Jan 23.
Zotero 插件