Wu Pei-Chun, Guo Lun-Zhang, Yu Shan, Zeng Ning, Liu Yu-Cheng, Yu Jia, Zhang Zhiming, Lu Ke, Sun Liangyu, Wang Chunfei, Chang Yu-Han, Lu Yin-Lin, Shen Yu-Fang, Tai Sheng, Chuang Yueh-Hsun, Ho Ja-An Annie, Huang Kai-Wen, Wu Yao-Ming, Liu Tzu-Ming
Institute of Translational Medicine, Faculty of Health Sciences & Ministry of Education Frontiers Science Center for Precision Oncology, University of Macau, Taipa, Macau, China.
Department of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Biomed Opt Express. 2022 Mar 10;13(4):1995-2005. doi: 10.1364/BOE.446749. eCollection 2022 Apr 1.
Using multiphoton fluorescent dosimetry, we demonstrate that the clearance dynamics of Indocyanine Green (ICG) in the blood can quickly reveal liver function reserve. In normal rats, the ICG retention rate was below 10% at the 15-minute post-administration; While in the rat with severe hepatocellular carcinoma (HCC), the 15-minute retention rate is over 40% due to poor liver metabolism. With a 785 nm CW laser, the fluorescence dosimeter can evaluate the liver function reserve at a 1/10 clinical dosage of ICG without any blood sampling. In the future, this low-dosage ICG 15-minute retention dosimetry can be applied for the preoperative assessment of hepatectomy or timely perioperative examination.
利用多光子荧光剂量测定法，我们证明了血液中吲哚菁绿（ICG）的清除动力学可以快速揭示肝功能储备。在正常大鼠中，给药后15分钟时ICG保留率低于10%；而在患有严重肝细胞癌（HCC）的大鼠中，由于肝脏代谢不良，15分钟时的保留率超过40%。使用785 nm连续波激光器，荧光剂量计可以在ICG临床剂量的1/10下评估肝功能储备，无需任何采血。未来，这种低剂量ICG 15分钟保留剂量测定法可用于肝切除术的术前评估或及时的围手术期检查。
