Moiseev Alexander A, Achkasova Ksenia A, Kiseleva Elena B, Yashin Konstantin S, Potapov Arseniy L, Bederina Evgenia L, Kuznetsov Sergey S, Sherstnev Evgeny P, Shabanov Dmitry V, Gelikonov Grigory V, Ostrovskaya Yuliya V, Gladkova Natalia D
Institute of Applied Physics Russian Academy of Sciences, 603155, 46, Ulyanova str., Nizhny Novgorod, Russia.
Privolzhsky Research Medical University, 603950, 10/1, Minin and Pozharsky sq., Nizhny Novgorod, Russia.
Biomed Opt Express. 2022 Mar 23;13(4):2393-2413. doi: 10.1364/BOE.457467. eCollection 2022 Apr 1.
A pilot post-mortem study identifies a strong correlation between the attenuation coefficient estimated from the OCT data and some morphological features of the sample, namely the number of nuclei in the field of view of the histological image and the fiber structural parameter introduced in the study to quantify the difference in the myelinated fibers arrangements. The morphological features were identified from the histopathological images of the sample taken from the same locations as the OCT images and stained with the immunohistochemical (IHC) staining specific to the myelin. It was shown that the linear regression of the IHC quantitative characteristics allows adequate prediction of the attenuation coefficient of the sample. This discovery opens the opportunity for the usage of the OCT as a neuronavigation tool.
一项初步尸检研究发现，从光学相干断层扫描（OCT）数据估算的衰减系数与样本的某些形态学特征之间存在很强的相关性，这些特征具体包括组织学图像视野中的细胞核数量以及该研究中引入的用于量化有髓纤维排列差异的纤维结构参数。这些形态学特征是从与OCT图像相同位置采集的样本的组织病理学图像中识别出来的，并用针对髓磷脂的免疫组织化学（IHC）染色进行染色。结果表明，IHC定量特征的线性回归能够充分预测样本的衰减系数。这一发现为将OCT用作神经导航工具提供了机会。
