冠状病毒前导RNA引发的转录：一种RNA剪接的替代机制。
Coronavirus leader-RNA-primed transcription: an alternative mechanism to RNA splicing.
作者信息
Lai M M
出版信息
Bioessays. 1986 Dec;5(6):257-60. doi: 10.1002/bies.950050606.
Abstract
Many viral and cellular mRNA species contain a leader sequence derived from a distant upstream site on the same gene by a process of RNA splicing. This process usually involves either nuclear functions or self‐splicing of RNA molecules. Coronavirus, a cytoplasmic RNA virus, unfolds yet another mechanism of joining RNA, which involves the use of a free leader RNA molecule. This molecule is synthesized and dissociates from the template RNA, and subsequently reassociates with the template RNA at down‐stream initiation sites of subgenomic mRNAs to serve as the primer for transcription. This leader‐primed transcriptional process thus generates viral mRNAs with a fused leader sequence. A similar mechanism might also operate in the mRNA transcription of African trypanosomes.
摘要
许多病毒和细胞mRNA种类都含有一个前导序列，该序列通过RNA剪接过程从同一基因上遥远的上游位点衍生而来。这个过程通常涉及核功能或RNA分子的自我剪接。冠状病毒是一种细胞质RNA病毒，展现出了另一种连接RNA的机制，该机制涉及使用一个游离的前导RNA分子。这个分子被合成并从模板RNA上解离，随后在亚基因组mRNA的下游起始位点与模板RNA重新结合，作为转录的引物。因此，这种前导引物转录过程产生了具有融合前导序列的病毒mRNA。类似的机制也可能在非洲锥虫的mRNA转录中起作用。
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