An Yixin, Su Haotian, Niu Qichen, Yin Shuxia
School of Grassland Science, Beijing Forestry University, Beijing, China.
Front Plant Sci. 2022 Apr 18;13:891361. doi: 10.3389/fpls.2022.891361. eCollection 2022.
Salt stress is among the most severe abiotic stresses in plants worldwide. is a model plant for legumes and analysis of its response to salt stress is helpful for providing valuable insights into breeding. However, few studies have focused on illustrating the whole-transcriptome molecular mechanism underlying salt stress response in . Herein, we sampled the leaves of treated with water or NaCl and analyzed the characteristics of its coding and non-coding RNAs. We identified a total of 4,693 differentially expressed mRNAs (DEmRNAs), 505 DElncRNAs, 21 DEcircRNAs, and 55 DEmiRNAs. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed that their functions were mostly associated with metabolic processes. We classified the lncRNAs and circRNAs into different types and analyzed their genomic distributions. Furthermore, we predicted the interactions between different RNAs based on the competing endogenous RNA (ceRNA) theory and identified multiple correlation networks, including 27 DEmiRNAs, 43 DEmRNAs, 19 lncRNAs, and 5 DEcircRNAs. In addition, we comprehensively analyzed the candidate DEmRNAs and ceRNAs and found that they were involved in Ca signaling, starch and sucrose biosynthesis, phenylpropanoid and lignin metabolism, auxin and jasmonate biosynthesis, and transduction pathways. Our integrated analyses in salt stress response in revealed multiple differentially expressed coding and non-coding RNAs, including mRNAs, lncRNAs, circRNAs, and miRNAs, and identified multiple DEmRNA and ceRNA interaction pairs that function in many pathways, providing insights into salt stress response in leguminous plants.
盐胁迫是全球范围内植物面临的最严重的非生物胁迫之一。（文中未提及具体植物名称，这里统一用“植物”指代）是豆科植物的模式植物，分析其对盐胁迫的反应有助于为育种提供有价值的见解。然而，很少有研究专注于阐明植物盐胁迫反应背后的全转录组分子机制。在此，我们采集了用水或氯化钠处理的植物叶片，并分析了其编码和非编码RNA的特征。我们共鉴定出4693个差异表达的mRNA（DEmRNAs）、505个差异表达的长链非编码RNA（DElncRNAs）、21个差异表达的环状RNA（DEcircRNAs）和55个差异表达的微小RNA（DEmiRNAs）。基因本体论和京都基因与基因组百科全书通路富集分析表明，它们的功能大多与代谢过程相关。我们将lncRNAs和circRNAs分类为不同类型，并分析了它们的基因组分布。此外，我们基于竞争性内源RNA（ceRNA）理论预测了不同RNA之间的相互作用，并鉴定出多个相关网络，包括27个DEmiRNAs、43个DEmRNAs、19个lncRNAs和5个DEcircRNAs。此外，我们全面分析了候选DEmRNAs和ceRNAs，发现它们参与了钙信号传导、淀粉和蔗糖生物合成、苯丙烷类和木质素代谢、生长素和茉莉酸生物合成以及转导途径。我们对植物盐胁迫反应的综合分析揭示了多种差异表达的编码和非编码RNA，包括mRNAs、lncRNAs、circRNAs和miRNAs，并鉴定出多个在许多途径中起作用的DEmRNA和ceRNA相互作用对，为豆科植物的盐胁迫反应提供了见解。
