Liu Junbo, Zhao Wensi, Liu Jin, Cai Xuhong, Liang Dadong, Tang Shanshan, Xu Bao
College of Resources and Environment, Key Laboratory of Straw Biology and Utilization, Ministry of Education, Jilin Agricultural University Changchun 130118 China
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences Beijing China.
RSC Adv. 2022 May 3;12(21):13235-13241. doi: 10.1039/d2ra01705a. eCollection 2022 Apr 28.
Quartz crystal microbalances (QCMs) have been widely used in the food industry, environmental monitoring, and biomedicine. Here, a molecularly imprinted QCM sensor was prepared and used for formaldehyde detection. Using polyvinyl chloride as the embedding material and tetrahydrofuran as the solvent, a QCM electrode was modified with HCHO molecularly imprinted polymers (HCHO-MIPs). The detection conditions of the sensor were optimized, and its selectivity was investigated. The theoretical calculation results revealed that the acrylamide and pentaerythritol triacrylate were potential candidate functional monomer and cross-linking agent, respectively, in the preparation of HCHO-MIPs with high adsorbability, superselectivity, and stability. According to the calculated results, a sensor had been prepared. When the pH was 7, the added mass of the HCHO-MIPs (or NIPs) was 20 mg, and the amount of PVC coating was 20 μL, the sensor exhibited good adsorption, selectivity, repeatability, high sensitivity, high accuracy, and a short response time. The lowest detection limit was 10.72 ng mL. The sensor exhibited higher selectivity for HCHO than for propionaldehyde and benzaldehyde. The HCHO contents in fresh shrimp samples were detected using the sensor for four cycles, and the detection rates were in the range of 97.56-98.60%. This study provided a theoretical and experimental basis for the rapid detection of HCHO.
石英晶体微天平（QCMs）已广泛应用于食品工业、环境监测和生物医学领域。在此，制备了一种分子印迹QCM传感器并用于甲醛检测。以聚氯乙烯为包埋材料，四氢呋喃为溶剂，用甲醛分子印迹聚合物（HCHO-MIPs）对QCM电极进行修饰。优化了传感器的检测条件，并研究了其选择性。理论计算结果表明，在制备具有高吸附性、超选择性和稳定性的HCHO-MIPs时，丙烯酰胺和季戊四醇三丙烯酸酯分别是潜在的功能单体和交联剂候选物。根据计算结果，制备了一种传感器。当pH值为7、HCHO-MIPs（或NIPs）的添加质量为20mg、PVC涂层量为20μL时，该传感器表现出良好的吸附性、选择性、重复性、高灵敏度、高精度和短响应时间。最低检测限为10.72 ng/mL。该传感器对甲醛的选择性高于丙醛和苯甲醛。使用该传感器对鲜虾样品中的甲醛含量进行了四个循环的检测，检测率在97.56-98.60%范围内。本研究为甲醛的快速检测提供了理论和实验依据。
