Teixeira Leonardo S G, Leão Elsimar S, Dantas Alaílson F, Pinheiro Heloísa L C, Costa Antonio C S, de Andrade Jailson B
Instituto de Quimica-Universidade Federal da Bahia, Campus Universitário de Ondina, 40.170-280-Salvador, Bahia, Brazil; Departamento de Engenharia e Arquitetura-Universidade Salvador-UNIFACS-Av. Cardeal da Silva 132, 40.220-141-Salvador, Bahia, Brazil.
Talanta. 2004 Oct 20;64(3):711-5. doi: 10.1016/j.talanta.2004.03.047.
In this work, a solid phase spectrophotometric method in association with flow injection analysis for formaldehyde determination has been developed with direct measurement of light-absorption in C(18) material. The 3,5-diacetyl-1,4-dihydrolutidine produced from the reaction between formaldehyde and fluoral P was quantitatively retained on C(18) support and the spectrophotometric detection was performed simultaneously at 412nm. The retained complex was quickly eluted from C(18) material with the eluent stream consisting of a 50% (v/v) ethanol solution. The results showed that the proposed method is simple, rapid and the analytical response is linear in the concentration range of 0.050-1.5mgL(-1). The limit of detection was estimated as 30mugL(-1) and the R.S.D. 2.2% using a sample volume of 625muL. The system presented an analytical throughput of 20 determinations per hour. The method was successfully applied in the determination of formaldehyde in ethanol fuel.
在本研究中，开发了一种结合流动注射分析的固相分光光度法用于甲醛测定，该方法通过直接测量C(18)材料中的吸光度来实现。甲醛与氟试剂P反应生成的3,5-二乙酰-1,4-二氢卢剔啶被定量保留在C(18)载体上，并在412nm处同时进行分光光度检测。保留的络合物用由50%（v/v）乙醇溶液组成的洗脱液流快速从C(18)材料上洗脱下来。结果表明，该方法简单、快速，在0.050 - 1.5mgL(-1)的浓度范围内分析响应呈线性。检测限估计为30μg/L，使用625μL的样品体积时相对标准偏差为2.2%。该系统的分析通量为每小时20次测定。该方法成功应用于乙醇燃料中甲醛的测定。
