Hendy-Ibbs P, Cox H, Evan G I, Watson J V
Br J Cancer. 1987 Mar;55(3):275-82. doi: 10.1038/bjc.1987.53.
The c-myc nuclear associated oncoprotein has been quantitated simultaneously with DNA in nuclei extracted from archival biopsies of uterine cervix neoplasia. The oncoprotein and DNA were measured fluorimetrically in a flow cytometer using a mouse monoclonal antibody (MYC 1-6E10) and propidium iodide. Normal biopsies exhibited higher oncoprotein levels than carcinomas (P less than 0.00001). Furthermore, the maximum fluorescence signal in the normal tissue occurred at a lower antibody concentration compared with tumour tissue. There was no correlation between oncoprotein levels and histological grade, stage of disease, age of the patients or prognosis in the carcinomas. Aneuploidy, defined as a distinct second peak separate from the diploid distribution, was not a significant feature. The c-myc oncoprotein nuclear content does not appear to be a prognostic indicator in carcinoma of the cervix from the results of these studies but there is clearly diagnostic potential, particularly for automated analysis of cervical screening.
在取自子宫颈瘤形成存档活检组织的细胞核中，已同时对c-myc核相关癌蛋白和DNA进行了定量分析。使用小鼠单克隆抗体（MYC 1-6E10）和碘化丙啶，在流式细胞仪中通过荧光法测量癌蛋白和DNA。正常活检组织的癌蛋白水平高于癌组织（P小于0.00001）。此外，与肿瘤组织相比，正常组织中的最大荧光信号出现在较低的抗体浓度下。癌组织中癌蛋白水平与组织学分级、疾病分期、患者年龄或预后之间无相关性。非整倍体（定义为与二倍体分布分开的明显第二个峰）不是一个显著特征。根据这些研究结果，c-myc癌蛋白的核含量似乎不是宫颈癌的预后指标，但显然具有诊断潜力，特别是对于宫颈筛查的自动化分析。
