Liu Juan, Weng Wenju, Xie Hui, Luo Guiling, Li Guangjiu, Sun Wei, Ruan Chengxiang, Wang Xianghui
Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
ACS Omega. 2019 Sep 11;4(13):15653-15659. doi: 10.1021/acsomega.9b02151. eCollection 2019 Sep 24.
In this paper, a hydroxyapatite (HAp)-doped carbon nanofiber (CNF)-modified carbon ionic liquid electrode (CILE) was prepared and used for the investigation on the direct electrochemistry and electrocatalysis of myoglobin (Mb). HAp nanoparticles were mixed within a polyacrylonitrile (PAN) solution, and a HAp@PAN nanofiber was synthesized by electrospinning process, which was further controlled by carbonization at 800 °C for 2 h in a nitrogen atmosphere to get a HAp@CNF nanocomposite. Various techniques were used to check the physicochemical properties of HAp@CNF. Mb was mixed with a HAp@CNF dispersion solution and casted on the surface of CILE to obtain an electrochemical sensing platform. The direct electrochemistry of Mb on the modified electrode was checked when a pair of enhanced redox waves appeared, indicating the direct electron transfer of Mb. HAp@CNF exhibited high conductivity, good biocompatibility, and large surface area, which was beneficial for Mb immobilization. The modified electrode showed excellent electrocatalytic activity toward the reduction of trichloroacetic acid and sodium nitrite, which was further used to establish a new electroanalytical method. Real samples were analyzed by the proposed method with satisfactory results.
本文制备了一种羟基磷灰石（HAp）掺杂的碳纳米纤维（CNF）修饰的碳离子液体电极（CILE），并用于研究肌红蛋白（Mb）的直接电化学和电催化性能。将HAp纳米颗粒混入聚丙烯腈（PAN）溶液中，通过静电纺丝工艺合成了HAp@PAN纳米纤维，然后在氮气气氛中于800℃碳化2h对其进行进一步处理，得到HAp@CNF纳米复合材料。采用多种技术对HAp@CNF的物理化学性质进行了表征。将Mb与HAp@CNF分散溶液混合并浇铸在CILE表面，以获得一个电化学传感平台。当出现一对增强的氧化还原波时，检测了Mb在修饰电极上的直接电化学，这表明了Mb的直接电子转移。HAp@CNF具有高导电性、良好的生物相容性和大表面积，有利于Mb的固定化。修饰电极对三氯乙酸和亚硝酸钠的还原表现出优异的电催化活性，并进一步用于建立一种新电分析方法。采用该方法对实际样品进行分析，结果令人满意。
