Environmental Engineering, The Catholic University of America, Washington, DC.
DC Water and Sewer Authority, Washington, District of Columbia.
Water Environ Res. 2019 Mar;91(3):185-197. doi: 10.1002/wer.1016. Epub 2019 Jan 30.
In this study, concurrent operation of anammox and partial denitrification within a nonacclimated mixed culture system was proposed. The impact of carbon sources (acetate, glycerol, methanol, and ethanol) and COD/NO3 -N ratio on partial denitrification selection under both short- and long-term operations was investigated. Results from short-term testing showed that all carbon sources supported partial denitrification. However, acetate and glycerol were preferred due to their display of efficient partial denitrification selection, which may be related to their different electron transport pathways in comparison with methanol. Long-term operation confirmed results of batch tests by showing the contribution of partial denitrification to nitrate removal above 90% after acclimation in both acetate and glycerol reactors. In contrast, methanol showed challenges of maintaining efficient partial denitrification. COD/NO3 -N ratio mainly controlled the rate of nitrate reduction and not directly partial denitrification selection; thus, it should be used to balance between denitrification rate and anammox rate. PRACTITIONER POINTS: The authors aimed to investigate the impact of carbon sources and COD/NO3-N ratio on partial denitrification selection. All the carbon sources supported partial denitrification as long as the nitrite sink was available. 90% partial denitrification could be achieved with both acetate and glycerol in long-term operations. COD/NO3-N ratio did not directly control partial denitrification but can be used to balance between denitrification rate and anammox rate.
在本研究中，提出了在未经驯化的混合培养系统中同时进行厌氧氨氧化和部分反硝化的方法。考察了不同碳源（乙酸盐、甘油、甲醇和乙醇）和 COD/NO3--N 比在短期和长期运行条件下对部分反硝化选择的影响。短期测试结果表明，所有碳源均支持部分反硝化。然而，由于乙酸盐和甘油在部分反硝化选择方面表现出高效性，因此它们是优选的碳源，这可能与其与甲醇相比具有不同的电子传递途径有关。长期运行结果通过在乙酸盐和甘油反应器中驯化后证实了分批测试的结果，即部分反硝化对硝酸盐去除的贡献超过 90%。相比之下，甲醇在维持高效部分反硝化方面存在挑战。COD/NO3--N 比主要控制硝酸盐还原速率，而不是直接控制部分反硝化选择；因此，应将其用于平衡反硝化速率和厌氧氨氧化速率之间的关系。 实践者要点：作者旨在研究碳源和 COD/NO3--N 比对部分反硝化选择的影响。只要有亚硝酸盐汇，所有碳源都支持部分反硝化。在长期运行中，乙酸盐和甘油都可以实现 90%的部分反硝化。COD/NO3--N 比不能直接控制部分反硝化，但可用于平衡反硝化速率和厌氧氨氧化速率。
