Guo Wangbiao, Cheng Jun, Song Yanmei, Kumar Santosh, Ali Kubar Ameer, Guo Caifeng, Qiao Zhanshan
State Key Laboratory of Clean Energy Utilization, Zhejiang University Hangzhou 310027 China
Ordos Jiali Spirulina Co., Ltd Ordos 016199 China.
RSC Adv. 2019 Jan 21;9(5):2746-2755. doi: 10.1039/c8ra09538h. eCollection 2019 Jan 18.
In order to solve the problems of the short residence time and low utilization efficiency of carbon dioxide (CO) gas added directly to a raceway pond, a CO bicarbonation absorber (CBA) was proposed to efficiently convert CO gas and sodium carbonate (NaCO) solution to sodium bicarbonate (NaHCO), which was dissolved easily in the culture medium and left to promote the microalgal growth rate. The CO gas reacted with the NaCO solution (initial concentration = 200 mM L and volume ratio in CBA = 60%) for 90 min at 0.3 MPa to give the optimized molar proportion (92%) of NaHCO product in total inorganic carbon and increase the microalgal growth rate by 5.0 times. Quantitative label-free protein analysis showed that the expression levels of the photosystem II (PSII) reaction centre protein () and PSII cytochrome () in the photosynthesis pathway increased by 4.8 and 3.4 times, respectively, while that of the RuBisCO enzyme () in the carbon fixation pathway increased by 3.5 times in cells cultivated with the NaHCO product in the CBA at 0.3 MPa.
为了解决直接添加到跑道式池塘中的二氧化碳（CO₂）气体停留时间短和利用效率低的问题，提出了一种CO₂碳酸氢化吸收器（CBA），以有效地将CO₂气体和碳酸钠（Na₂CO₃）溶液转化为碳酸氢钠（NaHCO₃），后者易于溶解在培养基中，从而促进微藻生长速率。CO₂气体在0.3MPa下与Na₂CO₃溶液（初始浓度 = 200 mM L且在CBA中的体积比 = 60%）反应90分钟，以使NaHCO₃产物在总无机碳中的摩尔比例达到最佳（92%），并使微藻生长速率提高5.0倍。无标记定量蛋白质分析表明，在光合作用途径中，光系统II（PSII）反应中心蛋白（）和PSII细胞色素（）的表达水平分别提高了4.8倍和3.4倍，而在0.3MPa下用CBA中的NaHCO₃产物培养的细胞中，碳固定途径中的核酮糖-1,5-二磷酸羧化酶（）的表达水平提高了3.5倍。
