Kaur Tanvir, Devi Rubee, Kumar Sunil, Sheikh Imran, Kour Divjot, Yadav Ajar Nath
Microbial Biotechnology Laboratory, Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, 173101, India.
Department of Genetics, Plant Breeding and Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, 173101, India.
Heliyon. 2022 Apr 25;8(4):e09326. doi: 10.1016/j.heliyon.2022.e09326. eCollection 2022 Apr.
Bioinoculants provide better opportunity for ecological farming practices to improve the plant growth and enhanced crop productivity. Different types of bioinoculants containing single microbial culture and multiple microbial strains in single formulation could be used for agricultural sustainability. The different efficient microbial strain in single formulation as a consortium is an emerging trend in the present era. The present study deals with the isolation of nitrogen fixing, phosphorus and potassium solubilizing microbes from rhizospheric soil and root's internal tissues of different cereal/pseudocereal crops and their application as a microbial consortium for the growth of cereal crops. A total of 152 rhizospheric and endophytic bacteria were isolated and screened for the plant growth promoting (PGP) traits of nitrogen fixation, solubilization of phosphorus, and potassium. Among all the isolates, nine were found to fix nitrogen, fifteen and eleven exhibited phosphorus and potassium solubilization activity, respectively. Three selected efficient bacterial strains were identified using 16S rRNA gene sequencing as sp. EU-B2SNL1 (N-fixer), EU-LWNA-37 (P-solubilizer), and EU-MRK-19 (K-solubilizer). The inoculation of these three bacterial strains on barley crop as single inoculum and as microbial consortium enhanced the growth and physiological parameters including root/shoot length and biomass, chlorophyll, carotenoids, phenolics, flavonoids and soluble sugar content in comparison with untreated control. The microbial consortium was found to be more effective as compared to single inoculum. The microbial consortium of nitrogen fixing and mineral solubilizing microbes could be used as biofertilizer for plant growth and soil health.
生物菌剂为生态农业实践提供了更好的机会，以促进植物生长并提高作物产量。不同类型的生物菌剂，包括单一微生物培养物和单一配方中的多种微生物菌株，可用于农业可持续发展。单一配方中作为联合体的不同高效微生物菌株是当今时代的一个新兴趋势。本研究涉及从不同谷类/伪谷类作物的根际土壤和根内部组织中分离固氮、解磷和解钾微生物，并将其作为微生物联合体用于谷类作物的生长。共分离出152株根际和内生细菌，并筛选其固氮、解磷和解钾等促进植物生长（PGP）特性。在所有分离物中，有9株被发现能固氮，15株和11株分别表现出解磷和解钾活性。通过16S rRNA基因测序鉴定出三株选定的高效细菌菌株，分别为sp. EU-B2SNL1（固氮菌）、EU-LWNA-37（解磷菌）和EU-MRK-19（解钾菌）。与未处理的对照相比，将这三株细菌菌株作为单一接种物和微生物联合体接种到大麦作物上，可提高其生长和生理参数，包括根/茎长度和生物量、叶绿素、类胡萝卜素、酚类、黄酮类和可溶性糖含量。结果发现，微生物联合体比单一接种物更有效。固氮和解矿质微生物的微生物联合体可作为生物肥料用于植物生长和土壤健康。
