Eshaghi Ebrahim, Mousae Sara, Hendiyani Ali, Habibi Khave Alireza, Nosrati Rahim
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Department of Biology, Nourdanesh Institute of Higher Education, Meymeh, Iran.
Iran J Microbiol. 2024 Dec;16(6):812-826. doi: 10.18502/ijm.v16i6.17260.
Plant growth-promoting rhizobacteria (PGPR) with a diverse set of traits can improve crop yield in agriculture. The current study aimed to evaluate the potential of multi-trait PGPR isolates as inoculants for maize growth.
In this study, 23 bacterial isolates were initially screened from maize plant rhizosphere. Ten isolates (A1-A10) were selected based on N fixation, P and K solubilization and their in vitro specific PGPR traits, such as solubilization of Zn, and Mn, the production of IAA, siderophore, ammonia, and HCN were assayed. Finally, the potential of selected isolates in enhancing the germination, height, shoot collar diameter, shoot fresh and dry weight biomass, and root dry weight of maize were evaluated.
Among the positive-PGPR colonies, the selected isolates demonstrated the better performance of PGPR traits such as highest nitrogen fixation, P, K, Mn, and Zn solubilization, and production of siderophore, HCN, NH3, and IAA. In addition, the maize seed germination and improvement of maize yield in a pot experiment were observed after their treatment by bacterial inoculants. Biochemical characteristics, 16S rDNA amplification, and sequencing demonstrated a high similarity of PGPR isolates to the strains of and .
The findings demonstrated that these strains could prove effective PGPR inoculants for the improvement of maize crops as multiple bio-fertilizers.
具有多种特性的植物促生根际细菌（PGPR）可提高农业作物产量。本研究旨在评估多性状PGPR分离株作为玉米生长接种剂的潜力。
本研究最初从玉米植物根际筛选出23株细菌分离株。基于固氮、磷钾溶解以及它们的体外特定PGPR特性，如锌和锰的溶解、吲哚乙酸（IAA）、铁载体、氨和氰化氢（HCN）的产生，选择了10株分离株（A1 - A10）进行测定。最后，评估了所选分离株在提高玉米发芽率、株高、茎基部直径、地上部鲜重和干重生物量以及根干重方面的潜力。
在阳性PGPR菌落中，所选分离株表现出较好的PGPR特性，如最高的固氮、磷、钾、锰和锌溶解能力，以及铁载体、HCN、NH₃和IAA的产生。此外，用细菌接种剂处理后，在盆栽试验中观察到玉米种子发芽和玉米产量的提高。生化特性、16S rDNA扩增和测序表明PGPR分离株与 和 的菌株具有高度相似性。
研究结果表明，这些菌株可作为有效的PGPR接种剂，作为多种生物肥料用于改良玉米作物。
