Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
Nanoscale. 2017 Sep 14;9(35):13187-13194. doi: 10.1039/c7nr04199c.
Lipid containing hybrid materials are of significant interest for biointerface research and drug delivery applications, and a large number of previous studies have focused on graphene oxide (GO). In this work, novel hybrid materials made of transition metal dichalcogenides (TMDCs) and phosphocholine (DOPC) liposomes were prepared and compared with GO. All these inorganic materials are 2D nanosheets. DOPC liposomes are adsorbed by tungsten disulfide (WS) as intact liposomes as indicated by cryo-TEM and liposome leakage assays. WS likely uses van der Waals forces for liposome adsorption as determined from urea, salt, and surfactant washing experiments. In addition, WS adsorbed doxorubicin (DOX) and DOPC liposomes synergistically. The adsorption capacity of DOPC on bare WS was 22.5% of the weight of WS. After adsorbing DOX on WS, the liposome adsorption capacity increased to ∼110%. Hydrogen bonding also contributed to liposome adsorption on DOX-loaded WS. Confocal fluorescence microscopy confirmed the uptake of the DOPC/WS hybrid material by HeLa cells, and the co-delivery of DOX and calcein was achieved by loading calcein inside the liposomes. This study provides fundamental insights into the interaction between PC liposomes and WS. Furthermore, preliminary biomedical applications of this hybrid material were explored.
含脂类的杂化材料在生物界面研究和药物输送应用中具有重要意义，大量先前的研究集中在氧化石墨烯（GO）上。在这项工作中，制备了由过渡金属二卤化物（TMDCs）和磷酰胆碱（DOPC）脂质体组成的新型杂化材料，并与 GO 进行了比较。所有这些无机材料都是 2D 纳米片。正如冷冻透射电镜和脂质体泄漏实验所表明的那样，DOPC 脂质体被二硫化钨（WS）吸附为完整的脂质体。WS 可能通过范德华力吸附脂质体，这是从尿素、盐和表面活性剂洗涤实验中确定的。此外，WS 协同吸附阿霉素（DOX）和 DOPC 脂质体。DOPC 在裸 WS 上的吸附量为 WS 重量的 22.5%。在 WS 上吸附 DOX 后，脂质体的吸附量增加到约 110%。氢键也有助于负载 DOX 的 WS 上的脂质体吸附。共聚焦荧光显微镜证实了 HeLa 细胞摄取 DOPC/WS 杂化材料，通过将 calcein 装载在脂质体内实现了 DOX 和 calcein 的共递送。这项研究为 PC 脂质体与 WS 之间的相互作用提供了基本的见解。此外，还探索了这种杂化材料的初步生物医学应用。
