Sun Jing, Wu Tao, Fan Qihang, Hu Qing, Shi Bin
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University Wuhan 430079 CN China
Department of Dental Implantology, School and Hospital of Stomatology, Wuhan University China.
RSC Adv. 2019 May 22;9(28):16106-16118. doi: 10.1039/c9ra00839j. eCollection 2019 May 20.
This study compared the effects of hydroxyapatite (HA), fluor-hydroxyapatite (FHA) and Si-substituted hydroxyapatite (SiHA) on osteogenic differentiation, osteoclastic activity and antibacterial properties. HA, FHA and SiHA were prepared a sol-gel reaction and characterized by scanning electron microscopic analysis (SEM), transmission electron microscopic analysis (TEM), and X-ray photoelectron spectrometry. Cell proliferation was evaluated using an MTT assay and cytoskeletal morphology was observed by fluorescence microscopy. Osteogenic differentiation was evaluated using alkaline phosphatase activity and Alizarin red staining. Quantitative real-time PCR was used to evaluate the mRNA expression of runt-related transcription factor 2 (Runx2) and osteopontin (OPN). New bone formation was tested using μCT, haematoxylin and eosin staining and TRAP staining. The antibacterial actions against (P. g) were evaluated through plate counting and live-dead bacterial staining. The results demonstrated that HA, FHA and SiHA can promote proliferation of bone mesenchymal stem cells (BMSCs). ALP activity in FHA extract with a concentration of 625 μg mL was the highest after 14 days osteogenic induction; similar results were observed for Runx2 and OPN mRNA expression. HA, FHA and SiHA decreased trabecular space in bone defects, but FHA reduced osteoclastic activity and inhibited P. g growth. In conclusion, FHA can promote osteogenic activity, reduce osteoclastic activity and enhance antibacterial effects.
本研究比较了羟基磷灰石（HA）、氟羟基磷灰石（FHA）和硅取代羟基磷灰石（SiHA）对成骨分化、破骨细胞活性和抗菌性能的影响。通过溶胶-凝胶反应制备HA、FHA和SiHA，并通过扫描电子显微镜分析（SEM）、透射电子显微镜分析（TEM）和X射线光电子能谱进行表征。使用MTT法评估细胞增殖，并通过荧光显微镜观察细胞骨架形态。使用碱性磷酸酶活性和茜素红染色评估成骨分化。采用定量实时PCR评估 runt相关转录因子2（Runx2）和骨桥蛋白（OPN）的mRNA表达。使用μCT、苏木精和伊红染色以及TRAP染色测试新骨形成。通过平板计数和死活细菌染色评估对牙龈卟啉单胞菌（P. g）的抗菌作用。结果表明，HA、FHA和SiHA可促进骨间充质干细胞（BMSC）的增殖。成骨诱导14天后，浓度为625 μg/mL的FHA提取物中的碱性磷酸酶活性最高；Runx2和OPN mRNA表达也观察到类似结果。HA、FHA和SiHA减少了骨缺损中的小梁间隙，但FHA降低了破骨细胞活性并抑制了牙龈卟啉单胞菌的生长。总之，FHA可促进成骨活性，降低破骨细胞活性并增强抗菌效果。
