College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, P. R. China.
Department of Radiotherapy, Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, 571199, P. R. China.
Chem Asian J. 2022 Jul 15;17(14):e202200388. doi: 10.1002/asia.202200388. Epub 2022 May 19.
Peroxynitrite (ONOO ) is one of the important reactive oxygen species, which plays a vital role in the physiological process of intracellular redox balance. Revealing the biological functions of ONOO will contribute to further understanding of the oxidative process of organisms. In this work, we designed and synthesized a novel red-emitting fluorescent probe MCSA for the detection of ONOO , which could rapidly respond to ONOO within 250 s and exhibited high sensitivity to ONOO with a low detection limit of 78 nM. Co-localization experiments demonstrated MCSA had the ability to localize into the mitochondria and endoplasmic reticulum. What's more, MCSA enabled monitoring ONOO level changes during tunicamycin-induced endoplasmic reticulum stress. We have also successfully achieved the visual detection of exogenous and endogenous ONOO in living cells and zebrafish. This work presented a chemical tool for imaging ONOO in vitro and in vivo.
过氧亚硝酸盐（ONOO−）是一种重要的活性氧物质，在细胞内氧化还原平衡的生理过程中起着至关重要的作用。揭示 ONOO−的生物学功能将有助于进一步了解生物体的氧化过程。在这项工作中，我们设计并合成了一种新型的红色荧光探针 MCSA 用于检测 ONOO−，它可以在 250s 内快速响应 ONOO−，并且对 ONOO−具有高灵敏度，检测限低至 78nM。共定位实验表明 MCSA 具有定位于线粒体和内质网的能力。更重要的是，MCSA 能够监测衣霉素诱导的内质网应激过程中 ONOO−水平的变化。我们还成功地实现了活细胞和斑马鱼中外源和内源性 ONOO−的可视化检测。这项工作为体外和体内成像 ONOO−提供了一种化学工具。
