Guanyun People's Hospital, Lianyungang 222000, China.
Modern Education Technology Center, Department of Critical Care Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, China.
Molecules. 2022 Jul 29;27(15):4858. doi: 10.3390/molecules27154858.
Peroxynitrite (ONOO), as an important reactive oxygen species (ROS), holds great potential to react with a variety of biologically active substances, leading to the occurrence of various diseases such as cancer and neurodegenerative diseases. In this work, we developed a novel mitochondria-localized fluorescent probe, HDBT-ONOO, which was designed as a mitochondria-targeting two-photon fluorescence probe based on 1,8-naphthylimide fluorophore and the reactive group of 4-(bromomethyl)-benzene boronic acid pinacol ester. More importantly, the probe exhibited good biocompatibility, sensitivity, and selectivity, enabling its successful application in imaging the generation of intracellular and extracellular ONOO. Furthermore, exogenous and endogenous ONOO products in live zebrafish were visualized. It is greatly expected that the designed probe can serve as a useful imaging tool for clarifying the distribution and pathophysiological functions of ONOO in cells and zebrafish.
过氧亚硝酸盐（ONOO−）作为一种重要的活性氧（ROS），有很大的潜力与各种生物活性物质发生反应，导致癌症和神经退行性疾病等各种疾病的发生。在这项工作中，我们开发了一种新型的线粒体定位荧光探针 HDBT-ONOO，它是基于 1,8-萘酰亚胺荧光团和 4-（溴甲基）-苯硼酸频哪醇酯的反应基团设计的线粒体靶向双光子荧光探针。更重要的是，该探针表现出良好的生物相容性、灵敏度和选择性，使其能够成功应用于成像细胞内和细胞外 ONOO 的产生。此外，还可视化了活斑马鱼中的外源性和内源性 ONOO 产物。预计设计的探针可以作为一种有用的成像工具，用于阐明 ONOO 在细胞和斑马鱼中的分布和病理生理功能。
